文献类型：期刊文献

英文题名：Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro

作者：Han, Hedan[1,2];Wang, Hai[1,2];Du, Yuemei[1,2];Gao, Liping[1,2]

第一作者：Han, Hedan

通讯作者：Gao, LP[1];Gao, LP[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Beijing Municipal Key Lab Biol Act Subst & Funct, Beijing 100191, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Municipal Key Lab Biol Act Subst & Funct, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：77

期号：4

起止页码：367-377

外文期刊名：CELL BIOCHEMISTRY AND BIOPHYSICS

收录：;Scopus(收录号：2-s2.0-85074703151);WOS:【SCI-EXPANDED(收录号:WOS:000492933300001)】；

基金：This work project is founded by grants from Scientific Research Project of Beijing Union University: Opening Project of Key Laboratory (NO.LDSP201801).

语种：英文

外文关键词：Oligomeric grape seed procyanidins; Cisplatin; HO-1; Nrf2; Nephrotoxicity

摘要：Cisplatin is a widely used anti-cancer drug. However, cisplatin is limited in clinical treatment because of its severe nephrotoxicity. This study reported whether O-GSP can antagonize the cisplatin-induced cytotoxicity in HEK293 cells through inducing HO-1 protein expression. We previously demonstrated O-GSP can increase the survival rate of HEK293 and have protective effect on HEK293 cells. Herein, We found that O-GSP can antagonize cisplatin nephrotoxicity through regulating the expression of HO-1. O-GSP promotes the translocation of Nrf2 in the nucleus, and activates the ERKN JNK pathway and p38 MAPK pathway. Interestingly, p38 MAPK plays a major role in HO-1 expression induced by O-GSP. And O-GSP can modulate the decrease of Nrf2 and HO-1 expression induced by cisplatin, and improve the cisplatin-induced activity and apoptosis rate of cells by stimulating the expression of HO-1. However, the protective effects of O-GSP are inhibited by ZnPP IX. Collectively, the results indicated that O-GSP induced the expression of HO-1 through p38MAPK and Nrf2 pathway in HEK293 cells.