文献类型：期刊文献

中文题名：有氧运动联合黑果枸杞对高脂膳食大鼠心肌脂代谢某些指标的影响

英文题名：Effects of aerobic exercise combined with Lycium ruthenicum on some indicators of myocardial lipid metabolism in rats with high-fat diet

作者：崔笑梅[1];曹建民[2];张静[3,4];周海涛[3,4];牛衍龙[5];胡戈[6];郭娴[2];李兆岩[3]

第一作者：崔笑梅

机构：[1]兰州理工大学,甘肃兰州730050;[2]北京体育大学,北京100084;[3]北京联合大学生物化学工程学院,北京100023;[4]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191;[5]赣南医学院,江西赣州341000;[6]长治医学院,山西长治046000

第一机构：兰州理工大学,甘肃兰州730050

年份：2020

卷号：36

期号：4

起止页码：301-305

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：北京市朝阳区协同创新项目(CYXC1817);北京市高等学校高水平人才交叉培养“实培计划”项目;北京联合大学科研项目(ZK70202005)。

语种：中文

中文关键词：有氧运动;黑果枸杞;高脂膳食;大鼠;心肌;脂代谢

外文关键词：aerobic exercise;lycium ruthenicum;high-fat diet;rat;myocardium;lipid metabolism

摘要：目的:研究有氧运动联合黑果枸杞对高脂膳食大鼠心肌脂代谢某些指标的影响。方法:55只雄性Wistar大鼠经过适应性饲养4 d后进行20 min/d的无负重游泳训练,连续3 d,筛选淘汰5只不适应游泳训练的大鼠后,按体重以数字随机分组法分为5组:普通膳食+安静组(RDC组)、高脂膳食+安静组(HDC组)、高脂膳食+黑果枸杞+安静组(HDLC组)、高脂膳食+有氧运动组(HDM组)、高脂膳食+黑果枸杞+有氧运动组(HDLM),每组10只。HDM组和HDLM组进行6周每周6次60 min/d的无负重游泳训练。RDC组大鼠以普通饲料常规喂养;其余各组以高脂饲料喂养;HDLC组和HDLM组大鼠灌胃黑果枸杞,灌胃剂量为4.48 g/(kg·d),灌胃体积为5 ml/kg,其余各组灌胃等量蒸馏水。6周后,测定大鼠Lee’s指数,取血、心肌检测相关生化指标。结果:与RDC组比较,HDC组Lee’s指数,血清FFA、TNF-α、IL-6、TC、TG、LDL-C,心肌FFA、ICAM-1显著升高(P<0.01);血清HDL-C水平显著降低(P<0.01)。与HDC组比较,HDLC、HDM、HDLM组Lee’s指数,血清FFA、TNF-α、IL-6、TC、TG、LDL-C,心肌FFA、ICAM-1显著降低(P<0.05或P<0.01);血清HDL-C水平显著升高(P<0.05或P<0.01)。与HDLC、HDM组比较,HDLM组Lee’s指数,血清FFA、TNF-α、IL-6、TC、TG、LDL-C,心肌FFA、ICAM-1显著降低(P<0.05);血清HDL-C水平显著升高(P<0.05)。结论:有氧运动和/或黑果枸杞干预能够不同程度改善高脂膳食大鼠脂代谢,降低肥胖引发的脂毒性。其中联合干预较单一干预更为有效。
Objective:To study the effects of aerobic exercise combined with Lycium ruthenicum on on some indicators of myocardial lipid metabolism in rats with high-fat diet.Methods:Fifty-five male Wistar rats were subjected to adaptive feeding for 4 days and weight-free swimming training for 3 days,20 min/d.After eliminating 5 rats that were not suitable for swimming training,the others were randomly divided into 5 groups according to their weight:regular diet+quiet control group(RDC),high fat diet+quiet control group(HDC),high-fat diet+Lycium ruthenicum quiet control group(HDLC),high fat diet+aerobic exercise group(HDM),high fat diet+Lycium ruthenicum+aerobic exercise group(HDLM),10 in each group.Group HDM and HDLM did 60 min/d swimming training for 6 weeks with no-bearing.Group C were fed regular diet;The other groups were fed with high-fat diet;Group HDLC and HDLM were intragastrically treated with Lycium ruthenicum at the dose of 4.48 g/(kg·d),and the volume was 5 mL/kg,and the other groups were given equivalent distilled water.The Lee's index,serum and myocardial biochemical indexes were measured after 6 weeks.Results:Compared with group RDC,Lee's index,serum free fatty acids(FFA),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),myocardial FFA and intercellular adhesion molecule-1(ICAM-1)increased significantly(P<0.01),serum level of high-density lipoprotein cholesterol(HDL-C)decreased significantly(P<0.01)in group HDC.Compared with group HDC,Lee's index,serum FFA,IL-6,TNF-α,TC,TG,LDL-C,myocardial FFA and ICAM-1 decreased significantly(P<0.05 or P<0.01),serum HDL-C levels increased significantly(P<0.05 or P<0.01)in group HDLC,HDM and HDLM.Compared with group HDLC and HDM,Lee's index,serum FFA,IL-6,TNF-α,TC,TG,LDL-C,myocardial FFA and ICAM-1 decreased significantly(P<0.05),serum HDL-C level increased significantly(P<0.05)in group HDLM.Conclusion:Aerobic exercise and/or Lycium ruthenicum can improve lipid metabolism in rats with high-fat diet,reduce lipotoxicity caused by obesity.Combined intervention is more effective.