文献类型：期刊文献

英文题名：Rapid screening and characterization of caffeic acid metabolites in rats by UHPLC-Q-TOF mass spectrometry

作者：Yuan, Jiaqi[1];Wang, Yunting[1];Mi, Shengquan[1];Zhang, Jiayu[2];Sun, Yaxuan[1]

第一作者：Yuan, Jiaqi

通讯作者：Sun, YX[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China;[2]Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China

第一机构：北京联合大学生物化学工程学院|北京联合大学应用文理学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2021

卷号：20

期号：2

起止页码：389-401

外文期刊名：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

收录：;Scopus(收录号：2-s2.0-85102820945);WOS:【SCI-EXPANDED(收录号:WOS:000625056400025)】；

基金：This work was financially supported by "National Key R&D Program of China" (nos. 2018YFC1311400 and 2018YFC1311405), Premium Funding Project for Academic Human Resources Development in Beijing Union University (no. BPHR2017CZ03), Academic Research Projects of Beijing Union University (no. XP202007), Postgraduate Research Innovation Support Program of Beijing Union University (no. YZ2020K001), the Visiting scholar research project of Beijing Union University, and Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University (no. 12213991724010239).

语种：英文

外文关键词：Caffeic acid; UHPLC-Q-TOF-MS/MS; Metabolic profiling

摘要：Purpose: To determine the metabolism of caffeic acid in rats. Methods: Sprague-Dawley rats were intragastrically administered caffeic acid in saline suspension, and biological samples collected. After sample pretreatment by solid phase extraction, ultra-high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry system (UHPLC-Q-TOF-MS/MS) was established to rapidly screen and characterize caffeic acid metabolites in rats. Waters HSS T3 UPLC chromatographic column (2.1 mm x 100 mm, 1.7 mu m) was applied for the gradient elution with aqueous solution of formic acid (A)-acetonitrile (B). Mass spectral data for the biological samples in electrospray positive and negative ion modes were collected and analyzed by SCIEX OS 1.3 workstation. Results: Based on their precise molecular weights and multistage mass spectrometry cleavage information, caffeic acid and 21 metabolites in vivo were identified. The results demonstrate that the biotransformation of caffeic acid in vivo was mainly achieved via hydrogenation, hydroxylation, methylation, sulfonation, glucuronidation, acetylation, and composite reactions. Conclusion: The metabolites and metabolic pathways of caffeic acid in rats have been rapidly elucidated, and its potential pharmacodynamics forms have been clarified. This provides a valuable and meaningful reference for the study of caffeic acid metabolites, biological activities, and its medicinal material basis in vivo.