文献类型：期刊文献

中文题名：淡豆豉、黄大豆及黑大豆中异黄酮苷元的提取与含量比较

英文题名：Extraction and Comparison of Isoflavone Aglycones in Seme Sojae Preparatum, Soybean and Black soybean

作者：张静[1];田平芳[1];葛喜珍[2];胡静远[2];李申[2];王鑫[1]

第一作者：张静

机构：[1]北京化工大学生命科学与技术学院;[2]北京联合大学生物化学工程学院

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2008

卷号：29

期号：4

起止页码：214-217

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：北京市教育委员会科技发展计划资助项目(KM200611417013);北京联合大学生化学院生物化工重点建设学科项目(0650411;0660415)

语种：中文

中文关键词：超声提取;聚酰胺;酶解;染料木素;大豆苷元;高效液相色谱

外文关键词：ultrosonic extraction： polyam ide; laydrolyze; genistein; daidzein; HPLC

摘要：为探讨淡豆豉、黄大豆及黑大豆中异黄酮苷元的提取与含量,本研究采用超声提取,用β-葡萄糖苷酶酶解大豆异黄酮中的糖苷键。以聚酰胺薄膜层析定性、HPLC定量检测不同原料中异黄酮苷元含量。实验结果表明,HPLC最佳流动相为甲醇-乙酸-水(12:1:10),染料木苷、大豆苷元和染料木素在20min内分离良好。大豆异黄酮苷元含量依次为:淡豆豉>黄大豆>黑大豆。精密度实验RSD分别为1.45%和1.6%,平均加样回收率100.93%,证明实验所建立的检测方法准确、可靠。
To explore the extraction and content of isoflavone aglycones in Seme Sojae Preparatum, soybean and black soybean, ultrosonic wave was used in the experiments. Besides, soybean isoflavone glucosides were transformed to aglycones by β - glucosidase. Soybean isoflavones in different materials were identified by the method of polyam ide TLC, while quantified by HPLC. The experiments results indicated that genistin, daidzein and genistein could be separated perfectly within twenty minutes under the mobile phase of methanol：aceticacid＇water （12：1：10）. The content of isoflavone aglycones was the most in Seine sojae Preparatum, and black soybean had the lest content. The precision RSD were 1,45 % and 1.6 % respectively, and the average recovery was 100.93 %, which proved that the method of experiments was both veracious and credible.