文献类型：期刊文献

英文题名：Lowing Effect of Wheat Germ Protein (WGP) on Cholesterol Level in Hypercholesterolemic HepG2 Cell

作者：Zhang, Yanzhen[1,2];Guan, Xin[1];Guo, Junxia[1,2];Zhang, Jing[1];Chen, Wen[1,2]

第一作者：张艳贞;Zhang, Yanzhen

通讯作者：Chen, W[1];Chen, W[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Food Sci Dept, Beijing 100023, Peoples R China;[2]Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Food Sci Dept, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：13

期号：2

起止页码：269-273

外文期刊名：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000473056800019)】；

基金：We acknowledge the financial supports of Beijing Natural Science Foundation (Grant Nos. 5153025, 5142004) and Premium Funding Project for Academic Human Resources Development in Beijing Union University (BPHR2018CZ01). We are also grateful to Professor Hulin Ren (Department of Foundational Courses, College of Biochemical Engineering, Beijing Union University) for reviewing and correcting the manuscript.

语种：英文

外文关键词：Wheat Germ Protein (WGP); Cholesterol; Bile Acid; HepG2 Cell; Real-Time PCR

摘要：Previous studies confirmed that wheat germ can significantly decrease plasma cholesterol. But which component is working still remains unclear except that wheat germ protein (WGP) may be important. This study examined the effect of WGP on cholesterol level of HepG2 cells and its possible mechanism. The results showed that compared with hypercholesterolemic cell (Hyper-c) group induced by water-soluble cholesterol, the levels of intracellular TC, FC and EC declined significantly (P < 0.05) after 48 h culture at or above the concentration of 0.04 mg/mL WGP. After 24 h treatment of 0.8 mg/mL WGP, intracellular TC and FC showed a significant downtrend (P < 0.05). After 24 to 48 h treatment of WGP, the intracellular bile acid quantity was gradually lowered (P < 0.05) while extracellular BA was gradually increased (P < 0.05). In contrast, the intracellular protein mass oppositely rose (P < 0.05) while extracellular was stable. All the mRNA levels of LDLR, CYP7A1, SREBP2 and LXR alpha were significantly up-regulated by WGP in comparison with Hyper-c group. These results indicate that WGP promotes the conversion of cholesterol to bile acid by up-regulating LXR alpha/CYP7A1 expression, finally impels bile acid transporting out of the cell.