文献类型：期刊文献

英文题名：Characterization of Pseudomonas stutzeri strain AY4 with nitrogen removal and application in wastewater treatment

作者：Fu, Weilai[1,2,3];Li, Xiaolong[2];Duan, Peifeng[3];Zhao, Youxi[1]

第一作者：Fu, Weilai

通讯作者：Zhao, YX[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing, Peoples R China;[2]Jiangsu Yuepeng Technol Co Ltd, Yancheng, Peoples R China;[3]Jiangsu Aishengmu Feed Co Ltd, Yancheng, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2025

外文期刊名：MICROBIOLOGY SPECTRUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001556575500001)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFC2100900) and the National Natural Science Foundation of China (32170031).

语种：英文

外文关键词：Pseudomonas stutzeri; ammonium and nitrate nitrogen removal; farm wastewater treatment; transcription level differences

摘要：A new strain capable of removing ammonium and nitrate nitrogen from animal farm wastewater was isolated and identified as Pseudomonas stutzeri strain AY4. Nitrogen balance analysis showed 52.9% and 63.2% of NH4+-N and NO3--N conversion to gaseous nitrogen, respectively, in laboratory conditions. Strain AY4 was also able to remove 92.7%, 95.8%, and 91.4% of total nitrogen, NH4+-N, and NO3--N in the real farm wastewater, which was significantly greater than that of the uninoculated control group. The quantitative real-time PCR experiment showed that the transcript levels of glnA, glnE, amtB, napA, narI, nirB, and nirD genes related to nitrogen metabolism were significantly upregulated during wastewater treatment. Strain AY4 showed high potential working in low carbon-to-nitrogen ratio and wide range of temperature adaptability, which makes it suitable for wastewater treatment in various environmental conditions. In addition, the study of transcript levels of genes related to nitrogen metabolism is important for revealing the mechanism of action of P. stutzeri in wastewater treatment.IMPORTANCEThe heterotrophic nitrifying-aerobic denitrifying bacterium Pseudomonas stutzeri strain AY4, isolated in this study, simultaneously removed ammonium nitrogen and nitrate nitrogen from wastewater. Upregulation of the transcript levels of glnA, glnE, amtB, napA, narI, nirB, and nirD genes associated with nitrogen metabolism favored the removal of nitrogen from actual wastewater. The isolation of novel strains with nitrogen removal ability has positively contributed to the treatment of nitrogen-containing farm wastewater.