文献类型：期刊文献

英文题名：Development of Artificial Riboswitches for Monitoring of Naringenin In Vivo

作者：Jang, Sungho[1];Jang, Sungyeon[1];Xiu, Yu[2,3,4];Kang, Taek Jin[5];Lee, Sang-Hyeup[6];Koffas, Mattheos A. G.[2,7];Jung, Gyoo Yeol[1,8]

第一作者：Jang, Sungho

通讯作者：Jung, GY[1];Jung, GY[2]

机构：[1]Pohang Univ Sci & Technol, Dept Chem Engn, 77 Cheongam Ro, Pohang 37673, Gyeongbuk, South Korea;[2]Rensselaer Polytech Inst, Dept Chem & Biol Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA;[3]Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China;[4]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100191, Peoples R China;[5]Dongguk Univ, Dept Chem & Biochem Engn, 30 Pildong Ro 1 Gil, Seoul 04620, South Korea;[6]Catholic Univ Daegu, Dept Life Chem, Hayang Ro 13-13, Gyongsan 38430, Gyeongbuk, South Korea;[7]Rensselaer Polytech Inst, Dept Biol Sci, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA;[8]Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, 77 Cheongam Ro, Pohang 37673, Gyeongbuk, South Korea

第一机构：Pohang Univ Sci & Technol, Dept Chem Engn, 77 Cheongam Ro, Pohang 37673, Gyeongbuk, South Korea

通讯机构：[1]corresponding author), Pohang Univ Sci & Technol, Dept Chem Engn, 77 Cheongam Ro, Pohang 37673, Gyeongbuk, South Korea;[2]corresponding author), Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, 77 Cheongam Ro, Pohang 37673, Gyeongbuk, South Korea.

年份：2017

卷号：6

期号：11

起止页码：2077-2085

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000416204100012)】；

基金：This work was supported by the Global Research Laboratory Program (NRF-2016K1A1A2912829) and the C1 Gas Refinery Program (NRF-2016M3D3A1A01913237) through the National Research Foundation of Korea (NRF) funded by the Korea Government (Ministry of Science, ICT & Future Planning).

语种：英文

外文关键词：naringenin; riboswitch; aptamer; riboswitch tuning; sensor-regulator; flavonoid

摘要：Microbial strains are considered promising hosts for production of flavonoids because of their rapid growth rate and suitability for large-scale manufacturing. However, productivity and titer of current recombinant strains still do not meet the requirements of industrial processes. Genetically encoded bio-sensors have been applied for high-throughput screening or dynamic regulation of biosynthetic pathways for enhancing the performance of microbial strains that produce valuable chemicals. Currently, few protein sensor-regulators for flavonoids exist. Unlike the protein-based trans-regulating controllers, riboswitches can respond to their ligands faster and eliminate off-target effects. Here, we developed artificial riboswitches that activate gene expression in response to naringenin, an important flavonoid. RNA aptamers for naringenin were developed using SELEX and cloned upstream of a dual selectable marker gene to construct a riboswitch library. Two in vivo selection routes were applied separately to the library by supplementing naringenin at two different concentrations during enrichments to modulate the operational ranges of the riboswitches. The selected riboswitches were responsive to naringenin and activated gene expression up to 2.91-fold. Operational ranges of the riboswitches were distinguished on the basis of their selection route. Additionally, the specificity of the riboswitches was assessed, and their applicability as dynamic regulators was confirmed. Collectively, the naringenin riboswitches reported in this work will be valuable tools in metabolic engineering of microorganisms for the production of flavonoids.