文献类型：期刊文献

英文题名：Production of 1,3-propanediol from glycerol using a new isolate Klebsiella sp AA405 carrying low levels of virulence factors

作者：Li, Ying[1,2];Ge, Xi-Zhen[2];Tian, Ping-Fang[1]

第一作者：Li, Ying;李映

通讯作者：Tian, PF[1]

机构：[1]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China

第一机构：Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing, Peoples R China.

年份：2017

卷号：31

期号：4

起止页码：725-732

外文期刊名：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000403940900009)】；

基金：This work was supported by grants from National High Technology Research and Development Program (863 Program) [grant number 2015AA021003]; National Basic Research Program of China (973 Program) [grant number 2012CB725200]; National Natural Science Foundation of China [grant number 21276014], [grant number 21476011]; Fundamental Research Funds for the Central Universities [grant number YS1407]; Innovation Capability Enhancement Program for universities Governed by Beijing Municipal Commission of Education [grant number PXM2015_014 209_000 010]; Beijing Natural Science Foundation [grant number 5164030].

语种：英文

外文关键词：Klebsiella pneumoniae; virulence factor; endotoxin; 1,3-propanediol; 2,3-butanediol

摘要：Klebsiella pneumoniae is a promising industrial species for the production of chemicals, yet its opportunistic pathogenicity restrains real-world applications. In this work, we identified a novel isolate designated Klebsiella sp. AA405 which carried low levels of virulence factors. No capsule, fimbriae and flagella were observed around colonies. Genome alignment between Klebsiella sp. AA405 and other 186 Klebsiella species revealed 3421 homologous genes. Notably, the endotoxin of Klebsiella sp. AA405 was only half of E. coli BL21. This strain matched well with common cloning and expression vectors and was sensitive to antibiotics. More importantly, this strain metabolized diverse carbon sources. Under micro-aerobic conditions, it produced 51.66 g/L 1,3-propanediol, 3.82 g/L 3-hydroxypropionic acid and 6.86 g/L 2,3-butanediol in a 5 L bioreactor using glycerol as the sole carbon source. Overall these results indicate that Klebsiella sp. AA405 is a promising strain for the production of bulk chemicals.