文献类型：期刊文献

中文题名：不同产地、不同储存期灸材对膝骨关节炎的药效比较

英文题名：Comparison of therapeutic effect of moxibustion materials from different places and storage periods on knee osteoarthritis

作者：孙建辉[1];霍海如[1];李小芹[1];李洪梅[1];康利平[2];张元[3]

第一作者：孙建辉

机构：[1]中国中医科学院中药研究所,北京100700;[2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700;[3]北京联合大学生物化学工程学院,北京100023

第一机构：中国中医科学院中药研究所,北京100700

年份：2020

卷号：45

期号：17

起止页码：4065-4070

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：国家重点基础研究发展计划(973)项目(2015CB554506);中央本级重大增减支项目(2060302);黔科合支撑(20172546)。

语种：中文

中文关键词：产地;储存期;艾叶;灸材;膝骨关节炎

外文关键词：origin;storage period;Artemisiae Argyi Folium;moxibustion material;knee osteoarthritis

摘要：骨关节炎(osteoarthritis,OA)是一种骨关节退行性病变,OA最常见的受累关节为膝、髋关节,OA发病率随年龄增长而增加,Meta分析显示艾灸治疗膝关节骨性关节炎(KOA)优于其他疗法。灸法功效的发挥与艾叶产地、储存年限相关,该研究比较了不同产地(湖北蕲春、河南南阳、湖南)、不同储存期(储存5年、3年、1年)灸材对膝骨关节炎大鼠模型的药效差异,测定了膝关节肿胀度、膝关节软骨病理组织学,并从Wnt/β-catenin信号通路角度探讨了艾灸治疗膝关节骨性关节炎的作用机制。研究表明湖北蕲春、河南南阳灸材组膝关节肿胀度明显低于模型组,差异有统计学意义(P<0.05),且优于湖南灸材组;与模型组相比,不同产地灸材组大鼠膝关节软骨组织细胞变性、病变有不同程度减轻,Mankin′s评分明显下降,湖北蕲春、河南南阳灸材组优于湖南灸材组。不同储存年限的灸材对大鼠骨关节炎关节肿胀度均有降低作用,蕲春5年组起效最早,治疗3 d后与模型组相比具有显著性差异;蕲春5年、3年灸材组病理组织学病变改善优于蕲春1年灸材组。作用机制研究显示,不同产地灸材对膝关节软骨β-连环蛋白(β-catenin)、骨形成蛋白-2(BMP-2)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶-13(MMP-13)基因的mRNA表达均具有降低作用,提示艾灸通过调控Wnt/β-catenin信号通路,抑制软骨基质降解,延缓骨关节炎软骨退变,这可能是艾灸治疗OA的作用机制之一。
Osteoarthritis(OA)is a kind of osteoarticular degenerative disease.The most common joint involvement of OA is knee and hip joint.The incidence rate of OA increases with age.Meta analysis shows that moxibustion is superior to other therapies in treating knee osteoarthritis(KOA).In this study,the effects of moxibustion materials from different places of origin(Hubei Qichun,Henan Nanyang,Hunan)and storage periods(5,3,1 years)on knee osteoarthritis in rats were compared.The swelling degree of knee joint and the histopathology of knee joint cartilage were measured.The mechanism of moxibustion in the treatment of knee osteoarthritis was discussed from the perspective of Wnt/β-catenin signaling pathway.The results showed that the swelling degree of knee joint in the moxibustion material group of Hubei Qichun and Henan Nanyang was significantly lower than that in the model group,the diffe-rence was statistically significant(P<0.05),and it was better than that in the moxibustion material group of Hunan Province;compared with the model group,the degeneration and pathological change of knee joint cartilage tissue in the moxibustion material group of different origins were alleviated to different degrees,the Mankin′s score was significantly reduced,and that in the moxibustion material group of Qichun in Hubei Province and Nanyang in Henan Province was significantly reduced and better than Hunan moxibustion material group.The effect of moxibustion materials with different storage years on the swelling degree of osteoarthritis in rats was the earliest in Qichun 5-year group,and there was significant difference between Qichun 5-year group and model group after 3 days of treatment;the improvement of histopathological changes in Qichun 5-year and 3-year moxibustion materials group was better than that in Qichun 1-year moxibustion materials group.The study on the mechanism of action showed that moxibustion materials from different places could reduce the mRNA expression ofβ-catenin,BMP-2,MMP-3,MMP-9 and MMP-13 genes in knee cartilage,suggesting that moxibustion could inhibit cartilage base by regulating Wnt/β-catenin signal pathway.It may be one of the mechanisms of moxibustion in the treatment of OA.