文献类型：期刊文献

中文题名：小檗碱和多菌灵复配对桃褐腐病菌的抑制效果

英文题名：Synergic inhibition of berberine with carbendazim against Monilinia fructicola

作者：符伟辉[1];葛喜珍[2];田平芳[1]

第一作者：符伟辉

机构：[1]北京化工大学生命科学与技术学院;[2]北京联合大学生物化学工程学院

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2013

卷号：40

期号：1

起止页码：89-92

中文期刊名：北京化工大学学报：自然科学版

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：北京市教委科技发展计划项目(km200611417013)

语种：中文

中文关键词：桃褐腐病菌;小檗碱;多菌灵;协同增效;共毒因子

外文关键词：Monilinia fructicola ; berberine ; carbendazim ; synergic effect ; co-toxicity coefficient

摘要：选用天然产物小檗碱和高效低毒的多菌灵进行复配,评价其对抑制桃褐腐病菌(Monilinia fructicola)的增效作用。采用菌丝生长速率法分别测定了小檗碱和多菌灵对桃褐腐病菌的抑制效果,经过线性回归计算得知其有效中浓度(EC50)分别为8.314μg/mL和0.012μg/mL。在此基础上,基于等效线法中的相加作用,设置6等分点复配比,以共毒因子作为评价增效标准,考察了复配对抑制桃褐腐病菌的增效效果。结果表明,当小檗碱和多菌灵复配浓度比为3657∶1时具有增效作用,即在小檗碱中加入微量多菌灵即能增强其抑菌效果。
Moniliniafructicola has developed resistance to the majority of fungicides predominant in the market. Given the probable synergic effect between fungicides, berberine was mixed with carbendazim in an effort to en- hance inhibition against M. fructicola. Firstly, the respective inhibition effects of berberine and carbendazim a- gainst M. fructicola were determined by measuring mycelium growth rates. By linear regression, the median effec- tive concentrations （ECho） were found to be 8. 314 μg/mL and 0. 012 μg/mL, respectively. Based on the equiva- lent linear method, six equal division points were used for determining the mixing ratio, and the synergic effect was evaluated by measuring co-toxicity coefficients. Astonishingly, a synergic effect occurred when the concentration ra- tio of berberine and carbendazim was 3657： 1, meaning that berberine inhibition against M. fructicola can be en- hanced when very little carbendazim is added.