文献类型：期刊文献

英文题名：Chemical characterization of the anti-inflammatory activity fraction of Epilobium angustifolium

作者：Lin, Pengcheng[1];Wang, Xin[2];Zhou, Na[2];Wu, Yong[2];Wang, Zeyu[2];Wu, Lei[1];Li, Jinjie[2];Shang, Xiaoya[2]

第一作者：Lin, Pengcheng

通讯作者：Shang, XY[1]

机构：[1]Qinghai Univ Nationalities, Coll Pharmaceut Sci, Key Lab Qinghai Tibet Plateau Phytochem Qinghai P, Xining 810000, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China

第一机构：Qinghai Univ Nationalities, Coll Pharmaceut Sci, Key Lab Qinghai Tibet Plateau Phytochem Qinghai P, Xining 810000, Peoples R China

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[114172]北京联合大学应用文理学院;[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;

年份：2022

卷号：248

期号：1

起止页码：35-44

外文期刊名：EUROPEAN FOOD RESEARCH AND TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000705134100001)】；

基金：The authors would like to acknowledge the research funding from the National Natural Sciences Foundation of China (NNSFC; Grant No. 81760783), the Innovation Platform for the Development and Construction of Special Project of Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province (2019-ZJ-Y19) and Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University (SWHX202005).

语种：英文

外文关键词：Epilobium angustifolium; Anti-inflammatory activity; UPLC-Orbitrap-MS; MS; Isolation and identification

摘要：The air-dried aerial part of Epilobium angustifolium is a very popular recreational tea in the Eastern edge of Europe. Studies have found that the extract of E. angustifolium has strong anti-inflammatory effects, and the oenothein B contained therein is speculated the main anti-inflammatory component. In this study, a fraction without oenothein B from the ethanol extract of E. angustifolium exhibited a much stronger anti-inflammatory activity than that of the oenothein B. Guided by rapid characterization of the chemical compositions based on UPLC-Orbitrap-MS/MS, a total of 39 compounds, including 20 flavonoids and 19 other phenolic acids were identified in the active fraction, based on the accurate mass within 3 ppm error. Further investigation resulted in the isolation and identification of 10 compounds, one of which was a new compound, and four known compounds were identified in E. angustifolium for the first time. All isolated compounds exhibited significant inhibitory activity on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW 264.7 cells. This study indicated that the anti-inflammatory active ingredients in E. angustifolium were mainly concentrated in the 30% ethanol eluted fraction obtained over macroporous adsorption resin. In addition to the oenothein B, there are a large number of other more effective anti-inflammatory components in E. angustifolium.