文献类型：期刊文献

英文题名：The CYP134 family in Gram-positive bacteria: From Bacillus to beyond, an orphan P450 lineage awaiting functional discovery

作者：Wang, Yiru[1,2];Li, Ying[1,2]

第一作者：Wang, Yiru

通讯作者：Li, Y[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2026

卷号：242

起止页码：9-21

外文期刊名：BIOCHIMIE

收录：;Scopus(收录号：2-s2.0-105024919521);WOS:【SCI-EXPANDED(收录号:WOS:001642453500001)】；

基金：This work is financially supported by the Project of Cultivation for Young Top-notch Talents of Beijing Municipal Institutions (BPHR202203209) .

语种：英文

外文关键词：Cytochrome P450; CYP134 family; Bacillus subtilis; Gram-positive bacteria; Orphan enzymes

摘要：Cytochrome P450 monooxygenases (CYPs) are pivotal enzymes in microbial metabolism, catalyzing a broad spectrum of oxidative reactions with ecological, physiological, and biotechnological significance. Within this superfamily, numerous Bacillus CYPs have been experimentally characterized, yet the CYP134 lineage remains poorly understood and is generally considered an orphan family. Although limited in vitro activities have been observed, a clear physiological substrate or function has not been defined. Despite this uncertainty, CYP134 members are widely distributed across Gram-positive bacteria, particularly within the Firmicutes, and exhibit the conserved structural motifs that safeguard P450 catalytic competence. Their expression is often responsive to environmental cues such as iron limitation and stress, pointing toward potential roles in specialized metabolic circuits and microbial competition. The evolutionary conservation, structural stability, and ecological responsiveness of these enzymes underscore their relevance as emerging candidates for functional discovery. This review integrates current structural, genomic, and ecological perspectives on CYP134 proteins, highlights unresolved questions, and proposes future avenues for exploring their biochemical activities and biotechnological applications. (c) 2025 Elsevier B.V. and Soci & eacute;t & eacute; Fran & ccedil;aise de Biochimie et Biologie Mol & eacute;culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies.