文献类型：期刊文献

中文题名：黑果枸杞对大鼠运动性心肌损伤的保护作用

英文题名：The Protective Effects of Lycium Ruthenicum Murr on the Exercise-induced Myocardial Injury in Rat

作者：崔纪芳[1];曹建民[2];周海涛[3];郭娴[2]

第一作者：崔纪芳

机构：[1]江苏师范大学;[2]北京体育大学运动人体科学学院;[3]北京联合大学生物化学工程学院

第一机构：江苏师范大学,江苏徐州221116

年份：2016

卷号：52

期号：5

起止页码：46-51

中文期刊名：中国体育科技

外文期刊名：China Sport Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;北大核心:【北大核心2014】；CSSCI:【CSSCI2014_2016】；

基金：北京市朝阳区协同创新项目(CYXC1508);国家体育总局科研课题(2013A101)

语种：中文

中文关键词：黑果枸杞;心肌损伤标志物;抗氧化酶;内皮素;降钙素基因相关肽;运动性心肌损伤

外文关键词：Lycium Ruthenicuzn Mutt. ; myocardial injury markers; antioxidant enzymes ET ;CGRP ; exercise-induced myocardial injury

摘要：目的:研究黑果枸杞对长时间、大强度运动导致的大鼠运动性心肌损伤的保护。方法:以大强度耐力训练大鼠为模型,将55只42天龄雄性SPF级Wistar大鼠分为5组(数字随机分组法):安静对照组(C组)、运动对照组(M组)、运动+黑果枸杞低剂量组(LM Ⅰ组),运动+黑果枸杞中剂量组(LM Ⅱ组),运动+黑果枸杞高剂量组(LM Ⅲ组),各组均为10只大鼠(剔除不符合实验要求的大鼠5只)。每天对大鼠灌胃(ig)1次,不同剂量水平干预组黑果枸杞剂量由低到高分别为0.25g/kg、0.5g/kg、1.5g/kg,灌胃体积为5 mL/kg,C组、M组大鼠ig等量生理盐水。游泳训练42天后,分别测定各组大鼠血清中谷丙转氨酶等心肌损伤标志物含量、心肌超氧化物歧化酶活性、丙二醛含量,血清、心肌内皮素及降钙素基因相关肽含量等相关生化指标。结果:血清谷丙转氨酶(P<0.01)、乳酸脱氢酶(P<0.01)、肌酸激酶(P<0.05)、a-羟丁酸脱氢酶(P<0.01)、肌钙蛋白Ⅰ(P<0.01),M组较C组显著升高。血清谷丙转氨酶、乳酸脱氢酶含量,LM各组较M组均有所降低(均为P<0.01),组间无明显差异;肌酸激酶含量,LM各组较M组均有所降低(LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.05、P<0.01),组间无差异;a-羟丁酸脱氢酶、肌钙蛋白Ⅰ含量,LM各组较M组均有所降低(LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.05、P<0.01),且组间随剂量增加而递减[LM Ⅲ组低于LM Ⅰ组(P<0.05)]。心肌SOD活性,M组较C组显著降低(P<0.01),LM各组较M组显著升高(LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.05、P<0.01),且组间随剂量增加而递增[LM II(P<0.05)、LM Ⅲ组(P<0.01)高于LM Ⅰ组]。心肌MDA含量,M组较C组显著性升高(P<0.05);LM各组较M组均显著降低(LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.05、P<0.01),且随黑枸杞剂量增加而递减[LM Ⅱ(P<0.05),LM Ⅲ组(P<0.01)低于LM Ⅰ组]。血清与心肌内皮素含量,M组较C组显著升高(P<0.05),LM各组较M组显著降低(血清内皮素LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.05、P<0.01,心肌内皮素LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.01、P<0.01),且组间皆随剂量增加而递减[LM Ⅲ组(P<0.05)低于LM Ⅰ组]。血清、心肌降钙素基因相关肽含量,M组较C组显著降低(P<0.05),LM各组较M组显著升高(LMⅠ、Ⅱ、Ⅲ组分别为P<0.05、P<0.05、P<0.01),且组间随剂量增加而递增[LM Ⅲ组(P<0.05)高于LM Ⅰ组]。结论:补充黑果枸杞对抗运动性心肌损伤具有多靶点、多途径的显著特点。不同剂量黑果枸杞的补充可以有效地促进抗氧化酶活性的增加,清除机体产生的过量的自由基,抑制内皮细胞分泌内皮素,促进降钙素基因相关肽的分泌,保证二者浓度的相对平衡。从而阻止因长时间、大强度运动导致的心肌脂质过氧化作用和心肌损伤,其中,以高剂量组(LMⅢ组)效果为最好。
Objective To study the protective effects of Lycium Ruthenicum Murr. after the long time and intensive exercise induced myocardial injury in rats. Methods： The model was based on intensive endurance training, 55 male Wistar rats at the age of 42-days old were divided into 5 groups randomly in test., control group （C group）, general training group （M group）, low dose Lycium Ruthenicum Murr. ＋ training group （LM I group）, middle dose Lycium Ruthenicure Murr. ＋ training group （LM II group）, high dose Lycium Ruthenicum Murr. ＋ training group （LM III group）（the rats that did not meet the requirements were removed）. Every group include 10 rats,and the rats were assigned to go on 42 days of swimming training. Pro- fessional garage were taken daily. The Rats in LM I, LM II and LM III group were given with 0. 25,0. 5 and 1.5 g/kg Lycium Ruthenicum Murr. garage at 5 mL/kg and other groups were given the normal saline. After 42 days of swimming training, we detected the content of myocardial injury markers like serum ALT, SOD and MDA, we also detected the biochemical indexes like serum and myocardial ET and CGRP. Results ： The content of serum ALT, LDH, CK ,a-HBDH and eTnI in M group were higher than C group （ALT,P〈0. 01,LDH,P〈0.01, CK,P〈0. 05,a-HBDH,P〈0.01,eTnI,P〈0. 01 ）. In the mean time, ALT and LDH in all LM group were lower than M group （P〈0.01） ,and CK in all LM group were lower than M group （LM I, P〈 0. 05, LM II , P〈 0. 05, LM III, P 〈 0. 01）, there were no differences between groups. The content of a-HBDH and cTnI in all LM group were lower than M group （LM I,P〈0.05,LM II ,P〈0.05,LM III,P〈0. 01）, and they were decreasing with the increase of Lyeium Ruthenicum Murr. Dose between groups （LM III was lower than LM I, P 〈0. 05）. The content of myocardial SOD（P〈0. 01）, both myocardial and serum C.GRP（P〈 0. 05） in M group were lower than C group , the content of myocardial MDA, myocardial and serum ET were higher than C group（P〈 0. 05）. The content of myocardial SOD, both myocardial and serum CGRP in all LM group were higher than M group （LM I, P〈0.05, LM II ,P〈0. 05,LM III,P〈0.01）. The content of myoeardial MDA, myocardial and serum ET were mueh lower than M group （MDA, serum ET： LM I, P〈0.05, LM If , P〈0. 05, LM III,P〈0. 01;myoeardial ET：LM I,P〈0.05,LM II ,P〈0.01,LM III,P〈0.01）. In the mean time, Myocardial SOD in LM II group, LM III group was much lower than LM I group （LM II ,P〈0. 05,LM III, P〈0. 01）. Both myocardial and serum CGRP in LM III group was much lower than LM I group （P 〈 0. 05 ）. With the increase of Lycium Ruthenieum Murr. dose, the increase of myocardial SOD, myocardial and serum CGRP. Myocardial MDA in LM II group, LM HI group was much higher than LM I group （LM II, P〈0. 05, LM III, P〈 0. 01）. Myocardial and serum ET in LM III group was much lower than LM I group （P 〈0. 05）. With the increase of Lycium Ruthenieum Murr. dose, the decrease of myocardial MDA and both myocardial and serum ET. Conclusion： The supplement of Lycium ruthenicum Murr. can protect exercise-induced myocardial injury in multiple targets and multiple ways. The different dose supplement will effectively keep the activity of antioxidant enzymes and scavenging the free radicals. In the mean time, it will inhibit the secretion of endothelin and promote the secretion of caleitonin gene related peptide, then ensure that the concentration of balance. At last, it will prevent the myocardial injury caused by long time and intensive training. The best dose-effect caused by Lycium ruthenicum Murr. was in high dose Lycium ruthenieum Murr. group.