文献类型：期刊文献

英文题名：Yak whey protein exerts anti-photoaging activity in HSF cells by regulating the MAPK/AP-1 signaling pathway

作者：Wang, Diandian[1,2];Zhou, Yaxi[3];Wang, Li[4];Li, Yang[1,2];Liu, Jiaqi[1,2];Yang, Tianyu[1];Song, Wei[2];Yan, Wenjie[1,2]

第一作者：Wang, Diandian

通讯作者：Yan, WJ[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100023, Peoples R China;[3]Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing 100093, Peoples R China;[4]Shunyi Dist Metrol & Food & Drug Inspect Ctr, Beijing 101300, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2026

卷号：30

外文期刊名：JOURNAL OF AGRICULTURE AND FOOD RESEARCH

收录：Scopus(收录号：2-s2.0-105047613809);WOS:【ESCI(收录号:WOS:001854119400001)】；

基金：This work was supported by the National Key Research and Development Program of China under Grant [2023YFF1103800] .

语种：英文

外文关键词：Yak whey protein; Photoaging; Human skin fibroblasts; MAPK/AP-1 signaling pathway

摘要：Chronic exposure to ultraviolet radiation disrupts the antioxidant defense system of the body, triggers inflammatory responses in the skin, and ultimately leads to photoaging. Yak whey protein is characterized by outstanding nutritional value and exhibits potent antioxidant and anti-aging biological activities. However, its anti-photoaging effects at the cellular level have not yet been elucidated. Therefore, this study established a photoaging model of human skin fibroblasts (HSF) via UVA irradiation, with the aim of investigating the potential anti-photoaging effects of YWP and its related molecular mechanisms. The results demonstrated that YWP exhibited significant protective effects against UVA-induced photoaging in HSF cells, which were associated with reduced reactive oxygen species (ROS), inflammatory cytokines, and matrix metalloproteinases (MMPs) via modulation of the MAPK/AP-1 pathway. Moreover, YWP promoted the production of hyaluronic acid, hyaluronic acid synthase 2, tissue inhibitor of metalloproteinases-2 (TIMP-2), and extracellular matrix (ECM) components. Among all groups, YWP at a concentration of 1.6 mg/mL exhibited the optimal therapeutic effect. Collectively, the findings obtained in this research underscore the promising anti-photoaging properties of YWP, while also offering both theoretical support and experimental evidence to facilitate the in-depth development and utilization of functional products originating from yak milk.