文献类型：期刊文献

英文题名：Insights into the interaction of DHA/EPA-containing phospholipids with modified whey protein isolate (mWPI) and chitosan quaternary ammonium salt (CQAS) to form microgel

作者：He, Yi[1];Heng, Jiangying[1];Zhang, Xian[2];Zhang, Zhihua[2];Sun, Hongshuai[3];Ma, Xuan[1];Zhang, Yu[1];Wang, Feng[1]

通讯作者：Wang, F[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]Minist Agr, China Green Food Dev Ctr, Beijing, Peoples R China;[3]Weifang Hairun Huachen Testing Technol Co Ltd, Weifang, Shandong, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2026

卷号：505

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20260319936824);Scopus(收录号：2-s2.0-105027640169);WOS:【SCI-EXPANDED(收录号:WOS:001674074300001)】；

基金：Funding This research was funded by National Key Research and Develop-ment Program (2023YFF1103804) , Major Science and Technology Project of Henan Province (No.231100310200) , and the Academic Research Projects of Beijing Union University (No. ZK20202516) .

语种：英文

外文关键词：DHA/EPA-containing phospholipids; Modified whey protein isolate; Chitosan quaternary ammonium salt; Microencapsulation; Composite microgel; Interpenetrating polymer network

摘要：This study developed a composite microgel system utilizing modified whey protein isolate (mWPI) and chitosan quaternary ammonium salt (CQAS) for encapsulating docosahexaenoic acid and eicosapentaenoic acidcontaining phospholipids (PL-DHA/EPA). Single-component systems exhibited limited encapsulation efficiencies of 15.50% (mWPI-PL) and 30.92% (CQAS-PL), attributed to high-pressure homogenization-induced protein denaturation and insufficient electrostatic interactions, respectively. The composite mWPI-CQAS-PL system achieved 88.75% encapsulation efficiency through synergistic electrostatic charge neutralization and interpenetrating polymer network formation, with positive zeta potential (+41.5 mV) ensuring colloidal stability and complete absence of phase separation over thirty days. Microstructural characterization revealed bi-layered particle architecture (approximately 10 mu m) featuring CQAS-rich outer shells and mWPI cores with uniform phospholipid distribution. Fluorescence spectroscopy evidenced protein-phospholipid interactions through excimer formation (480 nm) and microenvironmental polarity changes (430 nm), while molecular docking validated hydrogen bonding interactions. This mechanistic framework establishes design principles for advanced phospholipid delivery systems, significantly advancing marine lipid processing through innovative encapsulation strategies that preserve bioactivity while enhancing delivery performance.