文献类型：期刊文献

中文题名：绿僵菌与小檗碱复配制备水分散粒剂

英文题名：Preparation of water-dispersible granules by combination of Metarhizium anisopliae and berberine

作者：杨肖[1];葛喜珍[2];刘国新[3];田平芳[1]

第一作者：杨肖

机构：[1]北京化工大学生命科学与技术学院,北京100029;[2]北京联合大学生物化学工程学院,北京100023;[3]河北省化工研究院有限公司,石家庄050031

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2022

卷号：49

期号：1

起止页码：79-89

中文期刊名：北京化工大学学报：自然科学版

外文期刊名：Journal of Beijing University of Chemical Technology(Natural Science Edition)

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：河北省重点研发计划项目(19226509D/20322501D);北京市自然科学基金(KZ201911417049)。

语种：中文

中文关键词：小檗碱;绿僵菌;水分散粒剂;崩解时间;生物农药

外文关键词：berberine;Metarhizium anisopliae;water dispersible granule;disintegration time;biological pesticide

摘要：将金龟子绿僵菌(Metarhizium anisopliae)和小檗碱复配为水分散粒剂,可以起到既抗虫又抗病的双重效果,并且可降低田间施药的成本。测得小檗碱对绿僵菌的有效中浓度(EC_(50))为47.2 mg/mL,表明小檗碱对绿僵菌没有明显的抑制作用;根据以往绿僵菌的田间试验及小檗碱的抗真菌实验,确定小檗碱的最终添加量为3%(质量分数)。在单因素试验的基础上,利用响应面法确定水分散粒剂的配方为:25%绿僵菌孢子粉、3%小檗碱、3%乳化剂NP-10(润湿剂)、2.1%亚甲基双甲基萘磺酸钠(分散剂)、5.3%木质素磺酸钙(分散剂)、18%海藻酸钠(崩解剂)、1.7%羧甲基纤维素钠(黏结剂),用硅藻土(载体)补足100%(均为各成分占制剂的质量分数)。按照此配方在造粒机中制备了水分散粒剂,质量检测结果表明:所制备的水分散粒剂的崩解时间为174 s,孢子萌发率为85.4%,孢子悬浮率为86.34%,含水量为1.88%,杂菌率为2.01%,符合相关行业标准。
Combining Metarhizium anisopliae and berberine into water-dispersible granules offers the dual effects of insecticidal action and disease control, and can reduce the cost of field application. The median effective concentration(EC_(50)) of berberine against M. anisopliae was 47.2 mg/mL, indicating that berberine has no obvious inhibi-tory effect on M. anisopliae. According to previous field trials of M. anisopliae and antifungal experiments with berberine, the final amount of berberine was determined to be 3%(mass fraction). Based on single factor tests, the response surface method was used to determine the optimized formulation of the water-dispersible granules: 25% M. anisopliae spore powder, 3% berberine, 3% emulsifier NP-10(wetting agent), 2.1% sodium methylene dimethyl naphthalene sulfonate(dispersant), 5.3% calcium lignosulfonate(dispersant), 18% sodium alginate(disintegrant), 1.7% sodium carboxymethylcellulose(binder), and diatomaceous earth(carrier) as the remainder(the amount of ingredients refers to the mass fraction used in the preparation). Using this formulation, water-dispersible granules were prepared in a granulator. Quality test results showed that the disintegration time of the prepared water-dispersible granules was 174 s, the spore germination rate was 85.4%, the spore suspension rate was 86.34%, the water content was 1.88%, and the rate of microbial contaminants was 2.01%, which meets the relevant industry standards.