文献类型：期刊文献

中文题名：HPLC法同时测定唐松草多成分的含量

英文题名：Quantitative determination of multi-components in Thalictrum Baicalensis By HPLC

作者：程红霞[1];曾永箎[2];程维明[3]

第一作者：程红霞

机构：[1]北京联合大学生物化学工程学院;[2]浙江创新中药现代化研究所有限公司;[3]浙江省食品药品检验所

第一机构：北京联合大学生物化学工程学院

年份：2011

卷号：8

期号：3

起止页码：3-5

中文期刊名：北方药学

外文期刊名：Journal of North Pharmacy

语种：中文

中文关键词：唐松草;HPLC法;含量测定

外文关键词：T.Baicalensis HPLC content determination

摘要：目的:采用高效液相色谱法同时测定唐松草中的6个成分。方法:应用DiamonsilC18色谱柱,以乙腈—甲醇—0.05mol/L磷酸二氢钾溶液为流动相,梯度洗脱,柱温35℃,多波长同时检测。结果:6个成分的峰面积与浓度线性关系良好,加样回收率均符合要求。结论:该方法简单,准确可靠,重现性好,可用于该中药的质量控制。
Objective：To establish a HPLC method for the simultaneous determination of 6 components in Thalictrum Baicalensis.Method：Diamonsil C18 was used as the solid phase,and acetonitrile—methanol—0.05mol/L KH2PO4 solution as the mobile phase for the gradient elution.The oven temperature was 35℃,and the detective wavelengths were set as 220,274 and 348 nm.Results：the linearities of the 6 components were all good in the range of the test concentration,and the average recoveries were between 87.6% and 93.2%.Conclusion：the method was simple,reliable and reproducible,which could be used as the quality control method of T.Baicalensis.