文献类型：期刊文献

英文题名：Rapid Analysis of 18 Flavonoids in Tea by Ultrahigh-Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry

作者：Li, Jian[1,2];Ma, Junmei[3];Li, Qiang[3];He, Liangna[3];Zhang, Mingxing[3];Zheng, Lei[1];Zhang, Yan[3]

第一作者：Li, Jian;李健

通讯作者：Zheng, L[1];Zhang, Y[2]

机构：[1]Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China;[2]Beijing Union Univ, Coll Appl Arts & Sci, Beijing, Peoples R China;[3]Hebei Food Inspect & Res Inst, Hebei Engn Res Ctr Special Food Safety & Hlth, Hebei Food Safety Key Lab, Key Lab Special Food Supervis Technol State Market, Shijiazhuang, Peoples R China

第一机构：Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China

通讯机构：[1]corresponding author), Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China;[2]corresponding author), Hebei Food Inspect & Res Inst, Hebei Engn Res Ctr Special Food Safety & Hlth, Hebei Food Safety Key Lab, Key Lab Special Food Supervis Technol State Market, Shijiazhuang, Peoples R China.

年份：2023

卷号：2023

外文期刊名：JOURNAL OF FOOD QUALITY

收录：;EI(收录号：20233114456581);Scopus(收录号：2-s2.0-85165924715);WOS:【SCI-EXPANDED(收录号:WOS:001031413600001)】；

基金：AcknowledgmentsThis work was supported by the Major Science and Technology Projects in Anhui Province (Project no. 2020B06050001).

语种：英文

外文关键词：Liquid chromatography - Mass spectrometry - Methanol - Statistics

摘要：A method was established for the determination of 18 flavonoids in tea by ultra-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The tea samples were extracted by 70% (V/V) methanol aqueous solution, and separation was achieved on a Kinetex F5 column (2.1 mm x 100 mm, 2.6 & mu;m) with methanol and 0.1% formic acid in water as the mobile phase with a gradient elution. The samples were detected in TOF/MS and information-dependent acquisition (IDA)-MS/MS modes. The results showed that the relative standard deviations of 18 flavonoids were less than 5.0 ppm. The correlation coefficients (R-2) of the linear equation were greater than 0.998 in the range of 0.10-200 ng/mL. The limits of detection were 0.0010-0.040 ppm, and the limits of quantification were 0.0020-0.10 ppm. The recoveries ranged from 73.8% to 107% at spiked levels of 0.0020-1.0 ppm, with relative standard deviations (RSDs) being less than 10%. The method was simple, specific, and reliable. It could be used for the rapid screening and quantitative analysis of flavonoids in tea.