文献类型：期刊文献

中文题名：碱性果胶酶诱导黄瓜抗病机理的初步研究

英文题名：A preliminary study on the mechanism of induced disease resistance of cucumber by alkaline pectinase

作者：李祖明[1];范海延[2];白志辉[3];李鸿玉[1];张洪勋[3];荣瑞芬[1];叶磊[1]

机构：[1]北京联合大学师范学院,北京100011;[2]沈阳农业大学,沈阳110161;[3]中国科学院生态环境研究中心,北京100085

第一机构：北京联合大学师范学院

年份：2008

卷号：34

期号：5

起止页码：52-57

中文期刊名：植物保护

外文期刊名：Plant Protection

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：北京市优秀人才培养资助项目(20061D0502200295);国家自然科学基金(30600082)

语种：中文

中文关键词：克劳氏芽孢杆菌;碱性果胶酶;诱导抗病性;生理生化特性

外文关键词：Bacillus clausii; alkaline pectinase;induced disease resistance; physiological and biochemical characters

摘要：以克劳氏芽孢杆菌(Bacillus clausii)S-4碱性果胶酶诱导黄瓜黄化苗,研究了该激发子对黄瓜生理生化特性的影响,来探究碱性果胶酶诱导黄瓜抗病的机理。结果表明,黄瓜黄化苗经碱性果胶酶诱导后,过氧化物酶、多酚氧化酶和过氧化氢酶活性上升,可溶性蛋白和维生素C含量升高,丙二醛和游离脯氨酸含量下降,活性氧自由基产生速率受到抑制。可见,克劳氏芽孢杆菌S-4碱性果胶酶诱导黄瓜抗病作用与植物体内多种防御相关物质的诱导密切相关。
Alkaline pectinase from Bacillus clausii S 4 was tested for its ability to induce changes of physiological and biochemical characters in cucumber seedlings, attempting to unveil the mechanism of induced disease resistance. Af ter treatment with alkaline pectinase, the contents of peroxidase, polyphenol oxidase, catalase, soluble protein and Vc increased significantly; on the contrary, the contents of free proline, malonaldehyde and production velocity of re active oxygen radica decreased. These results indicated that the plant disease resistance induced by alkaline pectinase was associated with the elicitation of various defense related substances in cucumber seedlings.