文献类型：期刊文献

中文题名：核桃多糖研究进展

英文题名：Research Progress on Walnut Polysaccharide

作者：荣瑞芬[1];齐琳[1];苏晨[1];刘洋[1];王兴国[2]

第一作者：荣瑞芬

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]河北省(邢台)核桃产业技术研究院,河北临城054300

第一机构：北京联合大学生物化学工程学院

年份：2021

卷号：39

期号：3

起止页码：11-21

中文期刊名：食品科学技术学报

外文期刊名：Journal of Food Science and Technology

收录：CSTPCD;;EI(收录号：20212310471237);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：河北省科技计划项目(18963002D-6);核桃产业国家创新联盟(NAWI)资助项目;北京联合大学研究生科研创新后资助项目(YZ2020K001)。

语种：中文

中文关键词：核桃多糖;提取;分离纯化;制备;结构特性;生物活性

外文关键词：walnut polysaccharides;extraction;separation and purification;preparation;structural characteristics;biological activity

摘要：核桃是世界四大干果之一,营养与保健价值极高。近年来植物多糖的生物活性备受关注,研究也越来越深入,但有关核桃仁多糖的研究极少。核桃各组织部位都含多糖,为更好地开发核桃多糖营养健康作用,特别是核桃粕多糖提取利用,对近年来国内外核桃多糖的提取、分离纯化、测定、分子结构特性及生物活性研究文献进行了全面、深入的梳理与分析,获得了一系列对核桃多糖研究有意义的数据信息:超声和微波辅助热水提取工艺效果好,高于85℃和150 min的提取工艺、体积分数超过90%的乙醇沉淀多糖得率高;Sevag法脱蛋白,再分别用不同截留分子质量透析与DEAE-Sepharose、Sephadex纯化可获得分子质量在7015~133021 u的不同多糖;不同组织部位核桃多糖都含有葡萄糖、鼠李糖、半乳糖、木糖、阿拉伯糖和甘露糖,表明核桃多糖以酸性杂多糖为主,并呈现抗癌、抗氧化、降血糖、抑菌、预防老年痴呆等多种生物活性。核桃多糖研究对核桃产业链的提质增效具有重要的实践意义。
Walnut is one of the four major dried fruits in the world with high nutrition and health care value.In recent years,the biological activity of plant polysaccharides has been widely concerned,and the research is also gradually deepened,however,there is few study focused on walnut kernel polysaccharides.Polysaccharides are distributed in all parts of walnut tissues,in order to better develop the nutritional and health effects of walnut polysaccharides,especially the extraction and utilization of walnut meal polysaccharides,the domestic and oversea literatures on the extraction,separation,purification,determination,molecular structure characteristics and biological activity of walnut polysaccharides in recent years had been comprehensively sorted and analyzed,a series of meaningful data and information for walnut polysaccharide research were obtained:ultrasonic and microwave-assisted hot water extraction process had good effect.High yield of polysaccharides was obtained under the extraction technology with temperature higher than 85℃and 150 min extraction time,and ethanol volume fraction more than 90%.After deproteinization by Sevag method,different molecular weight cutoffs and purification with DEAE-Sepharose and Sephadex could obtain multiple polysaccharides with molecular weights ranging from 7015 to 133021 u.Walnut polysaccharides in different tissues all contained glucose,rhamnose,galactose,xylose,arabinose and mannose,which indicating that walnut polysaccharides were mainly acidic heteropolysaccharides,they also exhibited various biological activities such as anti-cancer,anti-oxidation,hypoglycemic,antibacterial,and prevention of Alzheimer's disease.The research on walnut polysaccharide might have important practical significance for improving the quality and efficiency of walnut industry chain.