文献类型：期刊文献

英文题名：The calibration model in potassium ion flux non-invasive measurement of plants in vivo in situ

作者：Xue, Lin[1,2,4]; Zhao, Dong-jie[1,4]; Wang, Zi-yang[1,4]; Wang, Xiao-dong[5]; Wang, Cheng[5]; Huang, Lan[1,3,4]; Wang, Zhong-yi[1,3,4]

第一作者：薛琳;Xue, Lin

通讯作者：Wang, Zhong-yi

机构：[1] College of Information and Electrical Engineering, China Agricultural University, Beijing, 100083, China; [2] College of Information Technology, Beijing Union University, Beijing, 100101, China; [3] Modern Precision Agriculture System Integration Research Key Laboratory of Ministry of Education, Beijing, 100083, China; [4] Key Laboratory of Agricultural Information Acquisition Technology [Beijing], Ministry of Agriculture, Beijing, 100083, China; [5] National Engineering Research Center for Information Technology in Agriculture, Beijing, 100097, China

第一机构：College of Information and Electrical Engineering, China Agricultural University, Beijing, 100083, China

年份：2016

卷号：3

期号：2

起止页码：76-82

外文期刊名：Information Processing in Agriculture

收录：EI(收录号：20180804811253);Scopus(收录号：2-s2.0-85016992188)

基金：This research was supported by the National Natural Science Foundation of China (61571443), the National Key Scientific Instrument and Equipment Development Projects (2011YQ080052), the Specialized Research Fund for the Doctoral Program of Higher Education (20130008110035), the Beijing Higher Education Young Elite Teacher Project (YETP1756) and the Special Fund of Fundamental Research Funds for the Central Universities (2013YJ008).

语种：英文

外文关键词：Calibration - Electrophysiology - Ion exchangers - Microelectrodes - Potassium

摘要：SIET (Self-referencing Ion Electrode Technique) provides a novel electrophysiological tool which can non-invasively measure the dynamic influxes and effluxes of ions caused by the diffusion along the concentration gradients in vivo. However, in this technique ion fluxes are converted to voltage signals using an ion selective microelectrode at a small amplitude of μV, which is easy to be interfered by the ambient noise. Hence, effective solutions to the suppression of noise and calibration of ion flux measurement system are very important for this method. A K+-selective microelectrode was constructed using liquid ion exchangers (LIX) to investigate ion transport over plant tissue. A standard concentration gradient which simulates plant living cells was produced by an electrode with a certain tip diameter, filled with a solution containing a known K+ concentration in 100?mmol/L. An ion diffusion simulation model was established. This model evaluated the performance of ion flux measurement system in accuracy and reliability by comparing the consistency of the measured value and the predicted curve. K+ fluxes were measured within 25?min at each measuring point of distance 10, 20, 30, 40, 50, 80, and 100?μm from the K+ source, respectively. It can be seen that the K+ fluxes changes little, which indicates that ion flux measurement system has a reliable stability. The study provides a theoretical basis for a new non-invasive ion flux measurement method creation and a new sensors design. ? 2016 China Agricultural University