文献类型：期刊文献

英文题名：Assessing the Microbial Communities in Four Different Daqus by Using PCR-DGGE, PLFA, and Biolog Analyses

作者：Ling, Yuxi[1];Li, Wenying[2];Tong, Tong[1];Li, Zuming[1];Li, Qian[1];Bai, Zhihui[3];Wang, Guijun[4];Chen, Jiahao[4];Wang, Yuguang[4]

第一作者：Ling, Yuxi

通讯作者：Li, ZM[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]Natl Univ Def Technol, Coll Liberal Arts & Sci, Changsha, Peoples R China;[3]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China;[4]Hunan Wuling Spirits Co Ltd, Changde, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2020

卷号：69

期号：1

起止页码：27-37

外文期刊名：POLISH JOURNAL OF MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85079609685);WOS:【SCI-EXPANDED(收录号:WOS:000521107100003)】；

基金：This paper was supported by Beijing Natural Science Foundation [grant number 6173033], Beijing Municipal Commission of Education [grant number PXM2013_ 014209_07_ 000082] and Beijing Union University Foundation [grant number 22213591113/001].

语种：英文

外文关键词：Microbial community; Daqu; PCR-DGGE; PLFAs; Biolog

摘要：Daqu made from raw wheat, barley or pea is used as an inoculum for the fermentation of Chinese Baijiu. In this study, the microbial communities of four different types of Daqus (sauce-flavor Waling Daqu, sauce and strong-flavor Baisha Daqu, strong-flavor Deshan Daqu, and light-flavor Niulanshan Daqu) were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), phospholipid fatty acid (PLFA) analysis, and Biolog EcoPlates analysis (Biolog). Clear differences were seen between the microbial communities of the four Daqus. PCR-DGGE showed differences in the number and brightness of bands between the Daqus, indicating the presence of unique bacterial species in Deshan Daqu, Waling Daqu, and Niulanshan Daqu. Lactobacillus sanfranciscensis, Bacillus thertnoamylovorans, and some unclassified bacteria were unique to Wuling Daqu, Deshan Daqu, and Niulanshan Daqu, respectively. Moreover, some bacterial species were observed in all four Daqus. A total of 26 PLFAs between C12 to C20 were detected from the four Daqus by PLFA analysis. Wuling Daqu had the highest total and fungal biomasses, Baisha Daqu had the highest bacterial biomass, and Niulanshan Daqu had the highest ratio of fungal biomass to bacterial biomass. The Biolog results indicated differences in the carbon source use and mode of the four Daqus, and also demonstrated that each Daqu had varying abilities to utilize different types of carbon sources. The cluster analysis of the three methods showed that the microbial communities of the four Daqus were different. This study also demonstrates the applicability of the three analytical methods in the evaluating of the microbial communities of Daqus.