文献类型：期刊文献

中文题名：水飞蓟提取物及复合制剂对乙醇中毒小鼠肝的保护作用

英文题名：Protective effects of Silybum thistle extract and related formulations on liver in mice with alcoholism

作者：王亭乔[1];任吴疆[1];吕梁彧[3];魏嵘[2];郭月逸[2];赵建[1]

第一作者：王亭乔

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100023;[3]东华大学化学化工与生物工程学院,上海201620

第一机构：北京联合大学生物化学工程学院

年份：2024

卷号：40

期号：7

起止页码：1009-1013

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

语种：中文

中文关键词：水飞蓟提取物;护肝;抗氧化能力;炎性因子

外文关键词：thistle extract;protect the liver;antioxidant capacity;inflammatory factors

摘要：目的探究水飞蓟提取物(SME)及其复合制剂对乙醇性肝损伤的保护作用。方法通过灌胃12mL·kg~150%乙醇建立乙醇肝损伤模型。将小鼠随机分为空白组(给予蒸馏水)、模型组(乙醇肝损伤模型)、SME低和高剂量组(分别给予0.06、0.20g·kg^(-1)SME)、SME+灵芝提取物(GLE)低和高剂量组(分别给予0.10、0.30g·kg^(-1)SME+GLE,SME:GLE=1:1)、水飞蓟丹参片(JAS)低和高剂量组(分别给予0.68、2.04g·kg^(-1)JAS),每组12只。用全自动生化分析仪测定法测定小鼠血清中谷丙转氨酶(GPT),用酶联免疫吸附试验法测定小鼠血清中白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的水平,用紫外分光光度计测定肝组织氧化应激指标[过氧化氢酶(CAT)和总超氧化物歧化酶(T-SOD)]。结果空白组、模型组、SME低和高剂量组、SME+GLE低和高剂量组、JAS低和高剂量组的T-SOD含量分别为(192.54±49.00)、(141.65±34.72)、(205.83±32.77)、(191.68±25.83)、(192.31±28.79)、(177.82±32.61)、(218.58±74.80)和(210.24±31.65)U·mg·prot,CAT含量分别为(37.78±5.73)、(28.92±8.44)、(44.12±11.52)、(41.41±9.15)、(47.01±10.48)、(41.63±8.95)、(47.14±8.91)和(48.29±10.06)U·mg^(-1),GPT含量分别为(47.61±13.00)、(97.84±26.00)、(62.33±18.92)、(51.84±17.91)、(70.77±28.00)、(58.00±21.27)、(52.28±18.78)和(45.55±9.27)U·L^(-1),IL-6水平分别为(21.03±1.52)、(28.43±5.75)、(21.90±3.24)、(21.23±1.55)、(22.26±2.58)、(21.24±2.91)、(22.17±4.14)和(21.14±3.02)pg·mL^(-1);模型组的上述指标与空白组比较,SME组、SME+GLE组和JAS低、高剂量组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.01)。空白组、模型组、SME低和高剂量组、SME+GLE低和高剂量组、JAS低和高剂量组的TNF-α水平分别为(28.07±7.72)、(69.02±16.34)、(40.29±8.94)、(48.84±10.17)、(41.91±14.96)、(40.07±12.75)、(50.72±11.44)和(45.05±11.34)pg·mL^(-1)。模型组与空白组比较,SME低和高剂量组、SME+GLE低和高剂量组、JAS低剂量组与模型组比较,在统计学上差异均有统计学意义(均P<0.01)。结论SME及其复合制剂可以提高小鼠肝的抗氧化水平、减轻小鼠肝的炎症,对于急性乙醇中毒导致的肝损伤具有一定的改善作用。
Objective To investigate the protective effect of silymarin extract(SME)and its complex preparation on ethanol liver injury.Methods An ethanol liver injury model was established by gavage of 12 mL·kg^(-1)50% ethanol.Male mice were divided into blank group(distilled water),model group(ethanol liver injury model),SME-L,-H groups(6,20 mg·mL^(-1)SME),SME+Ganoderma lucidum extract(CLE)-L,-H groups(10,30 mg·mL^(-1)SME+CLE,SME:CLE=1:1),Jian An Shi Silymarin Pueraria Mirifica and Tansy tablets(JAS)-L,-H groups(68,204 mg·mL^(-1)JAS),there were 12 mice in each group.The serum levels of glutamic-oxaloacetic transaminase(GOT)in mice were measured by fully automated biochemical analyzer assay;the serum levels of interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α)in mice were measured by enzyme-linked immunosorbent assay(ELASA);the hepatic tissue of oxidative stress indexes[catalase(CAT)and total superoxide dismutase(T-SOD)]were measured by ultraviolet spectrophotometer.Results The T-SOD activity in the blank group,model group,SME-L,SME-H,SME+GLE-L,SME+CLE-H,JAS-L and JAS-H groups were(192.54±49.00),(141.65±34.72),(205.83±32.77),(191.68±25.83),(192.31±28.79),(177.82±32.61),(218.58±74.80)and(210.24±31.65)U·mg·prot^(-1);CAT activity were(37.78±5.73),(28.92±8.44),(44.12±11.52),(41.41±9.15),(47.01±10.48),(41.63±8.95),(47.14±8.91)and(48.29±10.06)U·mg^(-1);CPT levels were(47.61±13.00),(97.84±26.00),(62.33±18.92),(51.84±17.91),(70.77±28.00),(58.00±21.27),(52.28±18.78)and(45.55±9.27)U·^(-1);IL-6 levels were(21.03±1.52)),(28.43±5.75),(21.90±3.24),(21.23±1.55),(22.26±2.58)(21.24±2.91),(22.17±4.14)and(21.14±3.02)pg·mL^(-1).Comparing the above indexes in the model group with the blank group,and comparing the above indexes in the SME-L,SME-H,SME+GLE-L,SME+GLE-H,JAS-L,JAS-H groups with the model group,the differences were statistically significant(all P<0.O1).The TNF-αlevels in blank,model,SME-L,SME-H,SME+CLE-L,SME+GLE-H,JAS-L and JAS-H groups werewere(28.07±7.72),(69.02±16.34),(40.29±8.94),(48.84±10.17),(41.91±14.96),(40.07±12.75),(50.72±11.44)and(45.05±11.34)pg·mL^(-1).Comparing the model group with the blank group,the SME,SME+CLE-L,-M and JAS,-M groups with the model group,the differences were statistically significant(all P<0.01).Conclusion Silybum marianum extract and its compound preparation can increase the antioxidant level and reduce the inflammation of mouse liver,and have a certain improvement effect on liver injury caused by acute ethanol poisoning.