文献类型：期刊文献

英文题名：The determination of lycopene Z-isomer absorption coefficient on C30-HPLC

作者：Huang, Jin[1,2];Hui, Bodi[1]

第一作者：Huang, Jin

通讯作者：Hui, BD[1]

机构：[1]Beijing Union Univ, Dept Food Sci, Beijing 100023, Peoples R China;[2]COFCO Nutr & Hlth Res Inst Co Ltd, Beijing, Peoples R China

第一机构：北京联合大学生物化学工程学院食品科学系

通讯机构：[1]corresponding author), Beijing Union Univ, Dept Food Sci, Beijing 100023, Peoples R China.|[11417146]北京联合大学生物化学工程学院食品科学系;[11417]北京联合大学;[1141726]生物化学工程学院;

年份：2020

卷号：8

期号：11

起止页码：5943-5952

外文期刊名：FOOD SCIENCE & NUTRITION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000578754400001)】；

基金：We would take this opportunity to express our sincere gratitude to Beijing Municipal Commission of Education for their financial support to this study (Grant No. KZ200511417020).

语种：英文

外文关键词：all E-isomer; evaporative light scattering detector; lycopene; specific absorption coefficient; Z-isomer

摘要：BothE- andZ-isomers of lycopene are encountered in nature. Although they were separated on C30-HPLC by the mobile phase consisting of CH3CN-MeOH (A) and MTBE (B), the quantification ofZ-isomers cannot be archived at present because either their commercially available reference samples are currently lacking, or they are unstable. In this study, both the specific and molar absorption coefficients of 5, 9, and 13Z-isomers in the mobile phase were determined on the analytical C30-HPLC-PDA-ELSD, and further verified on the preparative C30-HPLC-PDA. The specific and molar absorption coefficients of 5, 9, and 13Z-isomers were finally verified to beA1cm1% = 3,422 and epsilon(mol) = 183,717,A1cm1% = 2,183 and epsilon(mol) = 117,199, andA1cm1% = 1,119 and epsilon(mol) = 60,076, respectively, in the mobile phase. With these determined coefficients, the quantifications of 5, 9, and 13Z-isomers were able to be archived on C30-HPLC-PDA with the mobile phase applied in this investigation.