文献类型：期刊文献

中文题名：液相色谱-串联质谱测定豆芽中4种植物生长调节剂

英文题名：Determination of Four Plant Growth Regulators in Bean Sprouts by Liquid Chromatography-tandem Mass Spectrometry

作者：李健[1,2];郑磊[2]

第一作者：李健

机构：[1]北京联合大学应用文理学院,北京100191;[2]合肥工业大学食品科学与工程学院,安徽合肥230009

第一机构：北京联合大学应用文理学院

年份：2019

卷号：35

期号：1

起止页码：257-263

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；

基金：国家重点研发计划项目(2017YFC1600600)

语种：中文

中文关键词：豆芽;植物生长调节剂;液相色谱-串联质谱

外文关键词：bean sprouts;plant growth regulators;liquid chromatography-tandem mass spectrometry

摘要：豆芽中植物生长调节剂的滥用是影响其食用安全的主要因素。本文开发了高效液相色谱-串联质谱方法测定豆芽中3-吲哚乙酸、4-氯苯氧乙酸、6-苄基腺嘌呤、赤霉素,质谱检测采用多反应监测模式,采用外标法定量。样品经超声辅助提取后,加入Na Cl盐析,有机相经分散固相萃取净化后,进液相色谱-串联质谱检测。4种植物生长调节剂的检出限为5μg/kg,定量限(limit of quantitation,LOQ)为10μg/kg。以黄豆芽和绿豆芽为基质,分别做LOQ、2LOQ和10LOQ三个水平的添加试验,每个水平6个重复,方法的回收率范围为65.1%～109.8%,相对标准偏差(RSD)范围为1.49%～10.08%。为降低样品测定时假阳性问题,做多反应监测的同时做目标离子的增强子离子扫描。本方法可用于市售的豆芽样品中3-吲哚乙酸、4-氯苯氧乙酸、6-苄基腺嘌呤、赤霉素的监测,为保障消费安全提供技术支撑。
The abuse of plant growth regulator(s)in bean sprouts is a major factor that affects the safety of bean sprouts for consumption.In this study,a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS)method was developed for the determination of 3-indoleacetic acid,4-chlorphenoxyacetic acid,6-benzyl adenine and gibberellin in bean sprouts,in which multiple-reaction monitoring mode was employed for simultaneous detection of target analytes and external standards were used for quantification.Test samples were obtained for HPLC-MS analysis,through ultrasonic-assisted extraction,NaCl-induced salting-out,and purification by dispersive solid-phase extraction(to yield organic phase).The detection limit of the 4 plant growth regulators was 5μg/kg,and the limit of quantitation(LOQ)was 10μg/kg.The recovery was in range of 65.1%~109.8%and the relative standard deviation(RSD)was in range of 1.49%~10.08%for soybean sprouts and mung bean sprouts when the spiking experiments were performed at the levels of LOQ,2 LOQ and 10 LOQ with six replicates at each level.The recovery was.Enhanced product ion scan was performed along with the multiple-reaction monitoring in order to reduce false positive results during analysis.This method can be used to monitor the contents of 3-indole acetic acid,4-chlorphenoxyacetic acid,6-benzyl adenine and gibberellin in the commercial bean sprouts,and provides technical support for ensuring consumer safety.