文献类型：期刊文献

中文题名：锁阳中儿茶素的富集及含量测定

英文题名：Isolation and Determination of Catechin from Cynomorium songaricum Rupr.

作者：栾娜[1];常平[2];庄丽影[1];尚小雅[1]

第一作者：栾娜

机构：[1]北京联合大学应用文理学院;[2]首都师范大学生命科学学院

第一机构：北京联合大学应用文理学院

年份：2010

期号：20

起止页码：10644-10645

中文期刊名：安徽农业科学

外文期刊名：Journal of Anhui Agricultural Sciences

收录：北大核心:【北大核心2008】；

基金：北京市教委科技计划项目(KM200811417002)

语种：中文

中文关键词：锁阳;儿茶素;大孔吸附树脂;高效液相色谱

外文关键词：Cynomorium songaricum Rupr.; Catechin; Macroporous adsorbent resin; HPLC

摘要：[目的]建立从锁阳中富集儿茶素的方法,并对儿茶素含量进行测定。[方法]用50%乙醇对锁阳进行超声提取,提取物经大孔吸附树脂对儿茶素进行富集,并对儿茶素富集部位进行含量测定。HPLC工作条件为:色谱柱C18(250.0mm×4.6mm,5μm);流动相为乙腈∶0.2%乙酸15∶85(V/V);柱温为室温;流速1ml/min,检测波长280nm。[结果]锁阳中的儿茶素主要集中在35%乙醇洗脱部分;阴性对照品与样品分离良好,儿茶素的线性范围为0.005～0.040mg/ml(r=0.9999),回收率为98%,RSD为0.658%。[结论]用大孔吸附树脂可从锁阳提取物中高效地分离纯化富集儿茶素;HPLC检测方法可行,测定误差小,结果较准确,重现性好。
[Objective] To study the isolation and purification methods of catechin of Cynomorium songaricum Rupr.and establish a HPLC method for the determination of catechin.[Methods]The dried Cynomorium songaricum Rupr.powder was extracted with 50% EtOH and purified via column chromatography on macroporous adsorbent resin.Using the reversed-phase HPLC determine the content of catechin of above fraction.The qualification of HPLC：C18column（250.0 mm×4.6 mm,5 μm）,with mobile phase consisted of acetonitrile：0.2%acetic acid（V/V）（15∶ 85）.The column temperature was room temperature.The flow rate was 1 ml/min and the detection wavelength was 280 nm.[Results]Catechin is concentrated the 35% EtOH eluting fraction.The sample and the negative control were separated well by HPLC.The linear ranges of catechin was 0.005-0.040 mg/ml（r=0.999 9）.The average recoveries of catechin was 98%（RSD=0.658%）.[Conclusion] This method is simple and rapid to isolate and purify the catechin from Cynomorium songaricum Rupr.and,the feasible quantitative analysis method with small determinate error,accurate result and good reproducibility.