文献类型：期刊文献

英文题名：Prevention of STAT3-related pathway in SK-N-SH cells by natural product astaxanthin

作者：Sun, Shao-Qian[1];Du, Feng-Xiang[2];Zhang, Li-Hua[2];Gu, Fu-Ying[2];Deng, Yu-Lin[1];Ji, Yi-Zhi[2]

第一作者：Sun, Shao-Qian

通讯作者：Deng, YL[1];Ji, YZ[2]

机构：[1]Beijing Inst Technol, Sch Mat Sci & Engn, 5 Zhongguancun South St, Haidian Dist Beijing 100081, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：Beijing Inst Technol, Sch Mat Sci & Engn, 5 Zhongguancun South St, Haidian Dist Beijing 100081, Peoples R China

通讯机构：[1]corresponding author), Beijing Inst Technol, Sch Mat Sci & Engn, 5 Zhongguancun South St, Haidian Dist Beijing 100081, Peoples R China;[2]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2023

卷号：23

期号：1

外文期刊名：BMC COMPLEMENTARY MEDICINE AND THERAPIES

收录：;Scopus(收录号：2-s2.0-85178205649);WOS:【SCI-EXPANDED(收录号:WOS:001110709300001)】；

基金：This work was supported by grants from the Scientific Research Common Program of the Beijing Municipal Commission of Education (No. KM201911417013), the Premium Funding Project for Academic Human Resources Development in Beijing Union University (No. BPHR2019DZ04), the Scientific Research Common Program of the Beijing Municipal Commission of Education (No. KM202111417011) and the Venus Research Training Students Program (x202211417050).

语种：英文

外文关键词：Neuroblastoma; Astaxanthin; STAT3; Cell proliferation; Apoptosis; Cell migration inhibition; Cell invasion inhibition

摘要：PurposeNeuroblastoma (NB) is the most common solid malignancy in children. Despite current intensive treatment, the long-term event-free survival rate is less than 50% in these patients. Thus, patients with NB urgently need more valid treatment strategies. Previous research has shown that STAT3 may be an effective target in high-risk NB patients. However, there are no effective inhibitors in clinical evaluation with low toxicity and few side effects. Astaxanthin is a safe and natural anticancer product. In this study, we investigated whether astaxanthin could exert antitumor effects in the SK-N-SH neuroblastoma cancer cell line.MethodMTT and colony formation assays were used to determine the effect of astaxanthin on the proliferation and colony formation of SK-N-SH cells. Flow cytometry assays were used to detect the apoptosis of SK-N-SH cells. The migration and invasion ability of SK-N-SH cells were detected by migration and invasion assays. Western blot and RT-PCR were used to detect the protein and mRNA levels. Animal experiments were carried out and cell apoptosis in tissues were assessed using a TUNEL assay.ResultWe confirmed that astaxanthin repressed proliferation, clone formation ability, migration and invasion and induced apoptosis in SK-N-SH cells through the STAT3 pathway. Furthermore, the highest inhibitory effect was observed when astaxanthin was combined with si-STAT3. The reason for this may be that the combination of astaxanthin and si-STAT3 can lower STAT3 expression further than astaxanthin or si-STAT3 alone.ConclusionAstaxanthin can exert anti-tumor effect on SK-N-SH cells. The inhibitory effect was the higher when astaxanthin was combined with si-STAT3.