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High Production of 3-Hydroxypropionic Acid in Klebsiella pneumoniae by Systematic Optimization of Glycerol Metabolism  ( SCI-EXPANDED收录)  

文献类型:期刊文献

英文题名:High Production of 3-Hydroxypropionic Acid in Klebsiella pneumoniae by Systematic Optimization of Glycerol Metabolism

作者:Li, Ying[1,2];Wang, Xi[1];Ge, Xizhen[2];Tian, Pingfang[1]

第一作者:Li, Ying;李映

通讯作者:Tian, PF[1]

机构:[1]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China

第一机构:Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China

通讯机构:[1]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China.

年份:2016

卷号:6

外文期刊名:SCIENTIFIC REPORTS

收录:;WOS:【SCI-EXPANDED(收录号:WOS:000376787300001)】;

基金:This work was supported by grants from National High Technology Research and Development Program (863 Program) (No. 2015AA021003), National Basic Research Program of China (973 Program) (No. 2012CB725200), National Natural Science Foundation of China (No. 21276014, 21476011), Fundamental Research Funds for the Central Universities (YS1407), and the Innovation Capability Enhancement Program for universities Governed by Beijing Municipal Commission of Education (No. PXM2015_014209_000010).

语种:英文

摘要:3-Hydroxypropionic acid (3-HP) is an important platform chemical proposed by the United States Department of Energy. 3-HP can be converted to a series of bulk chemicals. Biological production of 3-HP has made great progress in recent years. However, low yield of 3-HP restricts its commercialization. In this study, systematic optimization was conducted towards high-yield production of 3-HP in Klebsiella pneumoniae. We first investigated appropriate promoters for the key enzyme (aldehyde dehydrogenase, ALDH) in 3-HP biosynthesis, and found that IPTG-inducible tac promoter enabled overexpression of an endogenous ALDH (PuuC) in K. pneumoniae. We optimized the metabolic flux and found that blocking the synthesis of lactic acid and acetic acid significantly increased the production of 3-HP. Additionally, fermentation conditions were optimized and scaled-up cultivation were investigated. The highest 3-HP titer was observed at 83.8 g/L with a high conversion ratio of 54% on substrate glycerol. Furthermore, a flux distribution model of glycerol metabolism in K. pneumoniae was proposed based on in silico analysis. To our knowledge, this is the highest 3-HP production in K. pneumoniae. This work has significantly advanced biological production of 3-HP from renewable carbon sources.

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