文献类型：期刊文献

英文题名：Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry

作者：Li, Jian[1,2];Ma, Junmei[3,4];Li, Qiang[3];Fan, Sufang[3];Fan, Lixin[3];Ma, Hongyu[3];Zhang, Yan[3,4];Zheng, Lei[1]

第一作者：李健;Li, Jian

通讯作者：Zheng, L[1];Zhang, Y[2];Zhang, Y[3]

机构：[1]Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China;[2]Beijing Union Univ, Coll Appl Arts & Sci, Beijing, Peoples R China;[3]Hebei Food Inspect & Res Inst, Hebei Food Safety Key Lab, Shijiazhuang, Hebei, Peoples R China;[4]Hebei Med Univ, Coll Forens Med, Hebei Key Lab Forens Med, Shijiazhuang, Hebei, Peoples R China

第一机构：Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China

通讯机构：[1]corresponding author), Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China;[2]corresponding author), Hebei Food Inspect & Res Inst, Hebei Food Safety Key Lab, Shijiazhuang, Hebei, Peoples R China;[3]corresponding author), Hebei Med Univ, Coll Forens Med, Hebei Key Lab Forens Med, Shijiazhuang, Hebei, Peoples R China.

年份：2021

卷号：8

外文期刊名：FRONTIERS IN NUTRITION

收录：;Scopus(收录号：2-s2.0-85121737172);WOS:【SCI-EXPANDED(收录号:WOS:000738287200001)】；

基金：Funding This work was supported by the National Key Research and Development Plan of China (2016YFD0401104).

语种：英文

外文关键词：ultra-performance liquid chromatography; quadrupole-time of flight mass spectrometry; tea; free amino acids; detection

摘要：The purpose of this research was to develop a simple, sensitive, and accurate method for simultaneous determination of 35 free amino acids using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Tea samples were extracted with boiling water bath, and then separated by XBridge BEH Amide column by gradient elution. The exact mass and MS/MS spectra of the target compound was detected under the TOF-MS and Information dependent acquisition (IDA)-MS/MS mode. The results demonstrated good linearity (R-2 > 0.9980) in the range of 0.5-1,000 ng/mL. The limits of detection (LODs) were 0.13-25.00 mg/kg and the limits of quantitation (LOQs) were 0.25-50.00 mg/kg. The recovery rate ranged from 70.1 to 105.1% with relative standard deviations (RSDs) <11% (n = 6). This research provides a targeted strategy for developing an analysis method for amino acids in tea.