文献类型：期刊文献

中文题名：蛇床子配伍维生素C对大鼠运动性肾脏缺血再灌注的保护作用

英文题名：Protective Effect of Fructus Cnidii Combined with Vitamin C on Exercised-induced Renal Ischemia-reperfusion Injury of Rats

作者：郭爱民[1];曹建民[2];周海涛[3]

第一作者：郭爱民

机构：[1]中国石油大学(北京);[2]北京体育大学;[3]北京联合大学生物化学工程学院

第一机构：中国石油大学(北京),北京102249

年份：2014

卷号：26

期号：3

起止页码：335-339

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

语种：中文

中文关键词：蛇床子;维生素C;运动性缺血再灌注

外文关键词：Fructus Cnidii; vitamin C ; exercise-induced ischemia-reperfusion

摘要：本文对蛇床子配伍维生素C对大鼠运动性肾缺血再灌注损伤的保护作用及机制进行了研究。试验中对大鼠以1.5 g/kg的剂量的蛇床子灌胃,100 mg/kg的维生素C腹腔注射56 d,并进行力竭游泳训练及血尿素氮等生化指标测定。结果显示,8周的过度训练导致大鼠运动性肾缺血"再灌注",肾组织组织病理学发生明显改变,肾功能受到严重损坏。血尿素氮和血清肌酐各组较对照组明显上升,与OM组相比FOM组、VCOM组、FVCOM组明显下降,分别为P<0.05、P<0.05、P<0.01;与VCOM组相比,FOM组、FVCOM组明显下降,分别为P<0.05、P<0.01。SOD活性各组较对照组明显降低,与OM组相比FOM组、VCOM组、FVCOM组明显上升,分别为P<0.05、P<0.05、P<0.01;与VCOM组相比,FOM组、FVCOM组明显上升,分别为P<0.05、P<0.01。MDA含量各组较对照组明显升高,与OM组相比FOM组、VCOM组、FVCOM组明显下降,分别为P<0.05、P<0.05、P<0.01;与VCOM组相比,FOM组、FVCOM组明显降低,分别为P<0.05、P<0.01。从而说明蛇床子和维生素C均可以增强SOD活性,清除氧自由基,减轻脂质过氧化作用,对运动性缺血再灌注肾脏具有保护作用。蛇床子作用优于维生素C。二者配伍使用效果更好。
In this study, the protective effect and mechanism of Fructus Cnidii combined with vitamin C on renal ischemi- a-reperfusion injury of rats was investigated. The rats was gavaged with 1.5 g/kg of Fructus Cnidii and intraperitoneal injected with 100 mg/kg of vitamin C for 56 days. Exhaustive swim training of the rats was carried out and the blood urea nitrogen and other biochemical indexes were measured. The results showed that 8 weeks of overtraining led to exereisedinduced renal ischemia-reperfusion in rats. In addition, the kidney histopathology of rats was significantly changed and renal function was severely damaged. Compared with the control group, the blood urea nitrogen and serum creatin/ne of each group increased significantly. Compared with OM group, FOM, VCOM and FVCOM groups decreased significantly （ P 〈 O. 05, P 〈 O. 05, P 〈 O. 01 ） ; compared with VCOM group, FOM and FVCOM groups decreased significantly （ P 〈 0. 05, P 〈 0.01 ）. Compared with the control group, the activity of SOD in each group was significantly decreased. Compared with OM group, FOM, VCOM and FV, COM groups increased significantly （P 〈 0.05, P 〈 0.05, P 〈 0.01 ） ; compared with VCOM group ,FOM and FVCOM groups increased significantly （P 〈 0.05 ,P 〈 0.01 ）. The content of MDA in each group was significantly higher than the control group. Compared with OM group, FOM, VCOM and FVCOM groups decreased significantly （ P 〈 0. 05, P 〈 0. 05, P 〈 0.01 ） ; compared with VCOM group, FOM and FVCOM groups decreased significantly （P 〈0.05 ,P 〈0.01 ）. These experimental results showed that the combination of Fructus Cnidii and vitamin C had a protective effect on exercise-induced renal ischemia-reperfusion injury of rats.