文献类型：期刊文献

英文题名：Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

作者：Zhang, Xian[1,2];Han, Rumeng[1];Bao, Teng[2];Zhao, Xiaojing[3];Li, Xiangfei[1];Zhu, Manchi[1];Yang, Taowei[1];Xu, Meijuan[1];Shao, Minglong[1];Zhao, Youxi[4];Rao, Zhiming[1]

第一作者：Zhang, Xian

通讯作者：Rao, ZM[1]

机构：[1]Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;[2]Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA;[3]ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China;[4]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 10023, Peoples R China

第一机构：Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China

通讯机构：[1]corresponding author), Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China.

年份：2019

卷号：18

期号：1

外文期刊名：MICROBIAL CELL FACTORIES

收录：;Scopus(收录号：2-s2.0-85070480679);WOS:【SCI-EXPANDED(收录号:WOS:000479067800001)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos.: 31500065, 21778024, 31870066), the National Key Research and Development Program of China (2018YFA0900304), Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIB-KF201902), the Science and Technology Innovation Team Foundation of Ningxia hui autonomous region (KJT2017001), the Key Research and Development Program of Ningxia hui autonomous region (2017BY069, 2019BCH01002), the General Project of Beijing Municipal Education Commission (SQKM201311417004), the National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-06).

语种：英文

外文关键词：Corynebacterium crenatum; Synthetic engineering; Biocatalysis; Acetoin; 2; 3-Butanediol

摘要：Background Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion and mutually transition between AC and 2,3-BD in their natural producing strains not only led to a low selectivity but also increase the difficulty of downstream purification. Therefore, synthetic engineering of more suitable strains should be a reliable strategy to selectively produce AC and 2,3-BD, respectively. Results In this study, the respective AC (alsS and alsD) and 2,3-BD biosynthesis pathway genes (alsS, alsD, and bdhA) derived from Bacillus subtilis 168 were successfully expressed in non-natural AC and 2,3-BD producing Corynebacterium crenatum, and generated recombinant strains, C. crenatum SD and C. crenatum SDA, were proved to produce 9.86 g L-1 of AC and 17.08 g L-1 of 2,3-BD, respectively. To further increase AC and 2,3-BD selectivity, the AC reducing gene (butA) and lactic acid dehydrogenase gene (ldh) in C. crenatum were then deleted. Finally, C. crenatum Delta butA Delta ldh SD produced 76.93 g L-1 AC in one-step biocatalysis with the yield of 0.67 mol mol(-1). Meanwhile, after eliminating the lactic acid production and enhancing 2,3-butanediol dehydrogenase activity, C. crenatum Delta ldh SDA synthesized 88.83 g L-1 of 2,3-BD with the yield of 0.80 mol mol(-1). Conclusions The synthetically engineered C. crenatum Delta butA Delta ldh SD and C. crenatum Delta ldh SDA in this study were proved as an efficient microbial cell factory for selective AC and 2,3-BD production. Based on the insights from this study, further synthetic engineering of C. crenatum for AC and 2,3-BD production is suggested.