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EGCG enhances cancer cells sensitivity under Co-60 gamma radiation based on miR-34a/Sirt1/p53  ( SCI-EXPANDED收录 CPCI-S收录)  

文献类型:会议论文

英文题名:EGCG enhances cancer cells sensitivity under Co-60 gamma radiation based on miR-34a/Sirt1/p53

作者:Kang, Qiaozhen[1];Zhang, Xiaomiao[1];Cao, Nana[1];Chen, Chen[1];Yi, Juanjuan[1];Hao, Limin[2];Ji, Yizhi[3];Liu, Xin[1];Lu, Jike[1]

第一作者:Kang, Qiaozhen

通讯作者:Lu, JK[1];Hao, LM[2]

机构:[1]Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;[2]Acad Mil Sci PLA China, Quartermaster Res Inst Engn & Technol, Beijing 100010, Peoples R China;[3]Beijing Union Univ, Biochem Engn Coll, Beijing 100010, Peoples R China

第一机构:Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China

通讯机构:[1]corresponding author), Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;[2]corresponding author), Acad Mil Sci PLA China, Quartermaster Res Inst Engn & Technol, Beijing 100010, Peoples R China.

会议论文集:10th International Symposium on Natural Products Chemistry and Applications (ISNPCA)

会议日期:2019

会议地点:Chillan, CHILE

语种:英文

外文关键词:Ionizing radiation; Radiation protection; Radiosensitization; EGCG; MiR-34a

摘要:Ionizing radiation (IR) resistance and toxicity to normal cells are the main problems in radiotherapy for cancer. In this study, we demonstrated that epigallocatechin gallate (EGCG) could inhibit effectively IR-induced damage to mouse normal hepatic cells AML-12, and improve dramatically the radiosensitivity of mouse hepatoma cells H22 to Co-60 gamma. In addition, the different effects of EGCG and underlying molecular mechanisms based on microRNA-34a (miR-34a) and apoptosis-related proteins were investigated by cells viability analysis, quantitative realtime PCR (qRT-PCR), Western blot and cells transfection. The results indicated EGCG played the key role of radiosensitization on H22 cells by activating the tniR-34a/Sirt1/p53 signaling pathway. Besides, EGCG could down-regulate the expression of anti-apoptotic protein Bcl-2, and up-regulate the expression of pro-apoptotic proteins Bait and Caspase-3 in H22 cells. Interestingly, EGCG showed contrary results on AML-12 cells. Therefore, radiation protection and radiosensitization of EGCG were associated with apoptosis regulated by miR-34a/Sirt1/p53 signaling pathway.

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