文献类型：期刊文献

英文题名：Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food

作者：Li Y.; Chi M.M.; Ge X.Z.

第一作者：Li Y.

机构：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization, Biochemical Engineering College, Beijing Union University, Beijing, 100023, China

第一机构：北京联合大学生物化学工程学院生物医药系

年份：2019

卷号：12

期号：2

起止页码：218-224

外文期刊名：International Journal of Agricultural and Biological Engineering

收录：Scopus(收录号：2-s2.0-85065170923)

语种：英文

外文关键词：Bioremediation; Carbendazim; Food; Hydrolase; Soil

摘要：Carbendazim (MBC) is an effective antifungal and antibacterial pesticide in agricultural applications. However, the MBC-contaminated soil and food are difficult to be restored. In this work, a novel MBC-hydrolase HY-1 encoded by gene hy-1 from an isolated MBC-degrading bacteria Bacillus amyloliquefaciens has been screened and identified. The 858 bp hydrolase gene was expressed in E. coli BL21 and the 32 kDa hydrolase HY-1 was purified. The purified HY-1 was able to catalyze MBC into 2-aminobenzimidazole (2-AB) without the need for any cofactors. Then bioremediation experiment was conducted and both the strain Car4 and cell crude extract of E. coli (pET-hy1) accelerated MBC degradation in soil. Moreover, purified HY-1 was available in removing MBC residue on the surface of cucumber. This work explored the possibility of microbial and enzymatic bioremediation on MBC-contaminated soil and food, provide a new way for bioremediation of pesticide contaminations. ? 2019, Chinese Society of Agricultural Engineering. All rights reserved.