文献类型：期刊文献

英文题名：Neuroprotective effect of liquiritin against focal cerebral ischemia/reperfusion in mice via its antioxidant and antiapoptosis properties

作者：Sun, Ya-Xuan[2,3];Tang, Yue[4,5];Wu, Ai-Li[4,5];Liu, Ting[6];Dai, Xue-Ling[2];Zheng, Qiu-Sheng[6];Wang, Zhi-Bin[1]

第一作者：Sun, Ya-Xuan;孙雅煊

通讯作者：Wang, ZB[1]

机构：[1]Beijing Inst Drug Control, Beijing 100035, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[3]Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China;[4]Chinese Acad Med Sci, Peking Union Med Coll, Fuwai Hosp, Beijing 100037, Peoples R China;[5]Chinese Acad Med Sci, Cardiovasc Inst, Beijing 100037, Peoples R China;[6]Shihezi Univ, Minist Educ, Sch Pharm, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832002, Peoples R China

第一机构：Beijing Inst Drug Control, Beijing 100035, Peoples R China

通讯机构：[1]corresponding author), Beijing Inst Drug Control, Beijing 100035, Peoples R China.

年份：2010

卷号：12

期号：12

起止页码：1051-1060

外文期刊名：JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH

收录：;Scopus(收录号：2-s2.0-78650137389);WOS:【SCI-EXPANDED(收录号:WOS:000284891600007)】；

语种：英文

外文关键词：liquiritin; mice; focal cerebral ischemia; reperfusion; antioxidant; anti-apoptosis

摘要：Our present study was conducted to investigate whether liquiritin (7-hydroxy-2-[4-[3,4,5-trihydroxy-6-(hydroxymethyl) oxan-2-yl] oxyphenyl]-chroman-4-one, 1), an active component of Glycyrrhiza uralensis Fisch., exerts a neuroprotective effect against focal cerebral ischemia/reperfusion (I/R) in male Institute of Cancer Research (ICR) mice. On the establishment of mice with middle cerebral artery occlusion (MCAO) for 2h and reperfusion for 22h, liquiritin at the doses of 40, 20, and 10mg/kg was administered before MCAO once a day intragastrically for a subsequent 3 days. Neurological deficits and infarct volume were measured, respectively. The levels of malondialdehyde (MDA) and carbonyl, activities of superoxide anion (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) and reduced glutathione/oxidized disulfide (GSH/GSSG) ratio in brain were estimated spectrophotometrically. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) and terminal deoxynucleotidyl transferase-mediated DuTP-biotin nick end labeling (TUNEL)-positive cells were detected by immunohistochemical analysis. Our results showed that the neurological deficits, infarct volume, and the levels of MDA and carbonyl decreased, the ratio of GSH/GSSG and the activities of SOD, CAT, and GSH-Px were compensatorily up-regulated, and 8-OHdG and TUNEL-positive cells decreased after 22h of reperfusion in liquiritin-treated groups. These findings suggest that liquiritin might be a potential agent against cerebral I/R injury in mice by its antioxidant and antiapoptosis properties.