文献类型：期刊文献

英文题名：Berberine as a natural compound inhibits the development of brown rot fungus Monilinia fructicola

作者：Hou, Dong-yao[1];Yan, Chun-qi[1];Liu, Hai-xi[1];Ge, Xi-zhen[2];Xu, Wei-jian[1];Tian, Ping-fang[1]

第一作者：Hou, Dong-yao

通讯作者：Tian, PF[1]

机构：[1]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China.

年份：2010

卷号：29

期号：9

起止页码：979-984

外文期刊名：CROP PROTECTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000281107700011)】；

基金：This study was financially supported by Project of Golden Bridge Program of Beijing Association for Science and Technology of China.

语种：英文

外文关键词：Berberine; Inhibition; Monilinia fructicola; Peach brown rot

摘要：Peach brown rot is a severe disease caused by ascomycete fungus Monilinia fructicola and is primarily controlled by chemical fungicides. However, the utilization of fungicides has caused many problems such as microbial resistance, environmental pollution, and fruit contamination. The growing concern for food safety requires alternative management methods that are safe to humans and benign to the environment. Here we report that the extract from Coptis chinensis (a Chinese herb named "Huang Lian") demonstrates a strong inhibition to M. fructicola. The 50% effective concentration (EC50) of C. chinensis extract to M. fructicola was only 0.91 mg/mL, while the EC(50)s to other fungi such as Botrytis cinerea and Alternaria solani were 14.09 mg/mL and 27.35 mg/mL, respectively. These results indicate that the extract of C. chinensis has a specific inhibition to M. fructicola. Subsequent partitioning of the ingredients in C. chinensis extract revealed that berberine, the conventional anti-bacterial alkaloid, was the predominant ingredient that exerted robust inhibition against M. fructicola. The EC50 and minimum inhibitory concentration (MIC) against M. fructicola were as low as 4.5 mu g/mL and 46.9 mu g/mL, respectively. Compared to berberine's analogs in C. chinensis such as palmatine, berberine tannate and jatrorrhizine, berberine showed the strongest inhibition against M. fructicola. This finding provided insight into the structure activity relationship between berberine and its analogs. Notably, not only can berberine prevent spore germination and hyphal growth, it also inhibits the activity of cutinase secreted by M. fructicola, implying the potential function of berberine in reducing the pathogenesis of M. fructicola. In addition, the strong in vivo inhibition of berberine against M. fructicola was observed with no visual cytotoxicity noted to peach fruits, even at berberine concentration of 400 mu g/mL. Note that this was much higher than its MIC value (46.90 mu g/mL). Overall berberine, as a natural compound, may be a promising candidate in control of brown rot. (C) 2010 Elsevier Ltd. All rights reserved.