文献类型：期刊文献

中文题名：虾青素和氨基葡萄糖对大鼠慢性骨关节炎干预作用的研究

英文题名：THE PREVENTIVE EFFECTS OF ASTAXANTHIN AND GLUCOSAMINE ON CHRONIC OSTEOARTHRITIS IN RATS

作者：张田[1];陈世杰[2];柴智[3];王一如[4];赵晓红[4,5];劳文艳[4,5]

第一作者：张田

机构：[1]航空总医院中国科学院北京转化医学研究院临床营养科;[2]北京赛升药业股份有限公司;[3]北京联合大学学报编辑部;[4]北京联合大学功能食品科学技术研究院;[5]北京联合大学生物活性物质与功能食品北京市重点实验室

第一机构：航空总医院中国科学院北京转化医学研究院临床营养科,北京100012

年份：2018

卷号：40

期号：6

起止页码：587-593

中文期刊名：营养学报

外文期刊名：Acta Nutrimenta Sinica

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：北京联合大学生物活性物质与功能食品北京市重点实验室开放课题(No.Zk70201501)

语种：中文

中文关键词：虾青素;氨基葡萄糖;骨关节炎;炎性反应;Ⅱ型胶原

外文关键词：astaxanthin;glucosamine;osteoarthritis;inflammation;type Ⅱ collagen

摘要：目的通过与氨基葡萄糖(glucosamine,GLU)的作用比较,探讨虾青素(astaxanthin,AST)对大鼠慢性骨关节炎(osteoarthritis,OA)的干预作用及其机制。方法用关节腔注射白陶土-鹿角菜胶诱发大鼠急性骨关节炎,结合跑步运动,建立大鼠慢性骨关节炎模型。造模前开始灌胃,分别为750mg/kg GLU、150mg/kg AST、无菌水(空白组、模型组)和2mg/kg双氯芬酸钠(diclofenac sodium,DS,阳性对照),直至6w跑台运动后结束。测量大鼠骨关节肿胀度;HE和番红-O-固绿(saffron-O-solid green, SOFG)染色法观察关节软骨组织学病理变化;ELISA法测定血清金属基质蛋白酶-3 (matrix metalloproteinases-3,MMP-3)、金属基质蛋白酶抑制剂-1(tissue inhibitor of metalloproteinases,TIMP-1)、诱导型NO合成酶(Inducible nitric-oxide synthase, iNOS)、IL-1β和TNF-α的含量;黄嘌呤氧化酶法测定血清中SOD活力;免疫组化法检测关节软骨Ⅱ型胶原的表达。结果与空白组相比,模型组大鼠关节肿胀增加;软骨表面粗糙,软骨基质中蛋白聚糖染色重度减退;血清中MMP-3、TIMP-1含量明显升高(P<0.01);iNOS活力明显升高(P<0.01);IL-1β和TNF-α含量明显增加(P<0.01);SOD活力明显降低(P<0.01);关节软骨Ⅱ型胶原含量明显减少(P<0.01)。与模型组相比,DS、AST和GLU均能减轻关节肿胀,明显抑制血清MMP-3、TIMP-1、TNF-α含量(P<0.01)和iNOS活力的增加(P<0.05),抑制SOD活力的降低(P<0.01);改善关节软骨Ⅱ型胶原含量的减少(P<0.05)。比较GLU与AST的作用,除AST的抗氧化作用较强外,其他均以GLU作用更明显,GLU能明显降低IL-1β的含量(P<0.01)而AST的作用并不显著(P> 0.05);SOFG染色显示GLU组蛋白聚糖染色缺失现象明显减轻,而AST组的改善作用不如GLU组的明显。结论 AST、GLU均对大鼠慢性骨关节炎具有明显的改善作用,GLU抗炎、改善基质中蛋白多糖和Ⅱ型胶原的含量的作用更明显,而AST抗氧化的作用更好。
Objective To investigate the preventive effect and its mechanism of astaxanthin(AST) on osteoarthritis(OA) in rats compared to glucosamine. Methods OA rat model was established by injecting kaolin and carrageen-λ into the articular cavity to induce acute osteoarticular injury, and then running on the treadmill to develope chronic osteoarticular damage. Rats were divided into five groups, preceded by intragastric administration of GLU(750 mg/kg), AST(150mg/kg), diclofenac sodium(DS, 2 mg/kg, positive control), and sterile distilled water(10 mL/kg, for control and model groups) until the end of exercise. Arthroncus was measured. Tissue sections of knee joints were stained with hematoxylin eosin(HE) and saffron-O-solid green(SOFG). The levels of matrix metalloproteinase-3(MMP-3), tissue inhibitor of metalloproteinase-1(TIMP-1), inducible nitric oxide synthase(iNOS), interleukin-1β(IL-1β), and tumor necrosis factor-α(TNF-α) in serum were detected with enzyme-linked immunosorbent assay(ELISA) kits. Superoxide dismutase(SOD) activity in serum was measured by xanthine oxidase and nitrate reductase methods. The expression of type Ⅱ collagen was detected by immunohistochemistry. Results Compared with the control group, the arthroncus was increased. The surface of the cartilage was rough and the protein-oligosaccharide staining in the cartilage matrix was decreased. Serum MMP-3 and TIMP-1 concentrations, iNOS activity and concentrations of IL-1β, NO and TNF-α were increased(P<0.01), while SOD activity and type Ⅱ collagen expression were significantly declined(P<0.01) in the model group. Compared with the model group, DS, AST and GLU could reduce arthroncus and inhibit the increase of MMP-3 and TIMP-1 contents(P<0.01), iNOS activity(P<0.05), TNF-α content, while increase serum SOD activity and type Ⅱ collagen contents(P<0.05). The preventive effects of AST on chronic OA was better than that of GLU only in anti-oxidative effect. Serum IL-1β contents in the GLU group was inclined(P<0.01), but that in AST group was not(P >0.05). GLU group could also show this improvement through SOFG staining inspection but AST was not. Conclusion Both AST and GLU have obvious preventive effects on chronic osteoarthritis, The effects of anti-inflammation and anti-degeneration articular cartilage are more obvious for GLU, while the antioxidant effect of AST is stronger.