文献类型：期刊文献

英文题名：Taurine reduces cholesterol through CYP7A1 in a calcineurin-dependent manner

作者：Guo, Junxia[1];Ou, Tong[1];Gao, Ya[1];Zhao, Yuxing[1];Zhang, Jing[1];Zhang, Yanzhen[1];Chen, Wen[1]

第一作者：郭俊霞

通讯作者：Chen, W[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[114172]北京联合大学应用文理学院;[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;

年份：2024

卷号：62

期号：10

起止页码：802-809

外文期刊名：INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001350757600003)】；

基金：Funding statement This work was supported by grants from the National Natural Science Foundation for Young Scholars of China (31401501) and the Academic Research Projects of Beijing Union University (No.ZKZD202304) .

语种：英文

外文关键词：Calcineurin; Cholesterol; MEK1/2; Taurine

摘要：Taurine (2-aminoethanesulfonic acid) could reduce serum and liver cholesterol concentrations via regulating expression and the activity of cholesterol 7 alpha-hydroxylase (CYP7A1). To investigate the possible role of calcineurin in taurine upregulating CYP7A1 expression in HepG2 cells under high cholesterol conditions. High cellular cholesterol conditions were achieved using 0.2 mM cholesterol in HepG2 cells. Calcineurin was decreased by FK506 and was depleted in CnA beta-/- cells. HepG2 cells were cultured in a taurine-containing medium for 24 or 48 h. The levels of total intracellular cholesterol were determined by an enzymatic method, and the expression levels of calcineurin, CYP7A1, and key transcriptional regulatory molecules were detected by western blotting. High-cholesterol resulted in increased CYP7A1 and calcineurin expressions. Taurine exhibited cholesterol-lowering effects regardless of low/high cholesterol conditions or calcineurin status. Taurine induced the expression of CYP7A1, which was abolished by inhibiting or deleting calcineurin. Taurine suppressed MEK1/2, p-c-Jun, and SHP-1, key molecules in one inhibitory pathway of CYP7A1 transcription. In contrast, suppression of MEK1/2 but not p-c-Jun or SHP-1 was reversed after completely knocking down calcineurin. Calcineurin is required for taurine's upregulation of CYP7A1 expression through inhibiting MEK1/2, which was partly responsible for taurine's cholesterol-lowering effect.