文献类型：期刊文献

英文题名：Determination of Catechin by High Performance Liquid Chromatography and Ultraviolet Spectrophotometer

作者：Huo, Qing[1];Hao, Jing[1];Shi, Rongjian[2]

第一作者：霍清

通讯作者：Huo, Q[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2013

卷号：25

期号：16

起止页码：8940-8942

外文期刊名：ASIAN JOURNAL OF CHEMISTRY

收录：;Scopus(收录号：2-s2.0-84887007944);WOS:【SCI-EXPANDED(收录号:WOS:000330234000024)】；

基金：This work was supported by Biochemical Engineering College of Beijing Union University Subject Construction Projects.

语种：英文

外文关键词：Catechin; HPLC; UV

摘要：Catechin exist widely in plants, which can be used as medicine and treated for cardiovascular disease, cancer and other features. This experiment established a quantitative method for the determination of catechin, so as to provide references for its development and utilization. The contents of catechin was determined by HPLC. The HPLC conditions were as follows:A Diamonsil C-18 column as separation column (250 mm x 4.6 mm i.d., 10 mu m), mobile phase of methanol-water-acetic acid (V:V:V = 15:84:1), flow rate of 0.9 mL/min, detection wavelength of 278 nm and column temperature of 30 degrees C, HPLC standard curve:y = 555111x + 248469, R-2 = 0.9982, the linear relationship between catechin concentration and peak area was good in the range of 3.24-6.43 mu g, the recovery rates of the catechin was 100.33 % with RSD of 1.44 %. The contents of catechin were determined by UV spectrophotometer and UV conditions were as follows: Ethanol as a constant volume solvent, the absorbance of catechin was measured at 278 nm. Determination of catechin standard curve was y = 0.05x, R-2 = 1, linear range was 0.05-0.4 mg/mL.