文献类型：期刊文献

英文题名：In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

作者：An, Jun[1,2,3];Yang, Cui[1,2];Li, Zuming[1,2];Finn, Patricia W.[4];Perkins, David L.[4];Sun, Jun[4];Bai, Zhihui[5];Gao, Liping[1,2];Zhang, Michael[6];Ren, Difeng[3]

第一作者：An, Jun

通讯作者：Li, ZM[1];Li, ZM[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Beijing, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China;[3]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing Key Lab Forest Food Proc & Safety, Beijing 100083, Peoples R China;[4]Univ Illinois, Dept Med, Chicago, IL USA;[5]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China;[6]Univ Manitoba, Dept Phys & Astron, Winnipeg, MB R3T 2N2, Canada

第一机构：北京联合大学生物化学工程学院食品科学系

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Beijing, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China.|[114172]北京联合大学应用文理学院;[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;[11417146]北京联合大学生物化学工程学院食品科学系;

年份：2019

卷号：103

期号：2

起止页码：917-927

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000457830000030)】；

基金：This work was supported by the China Scholarship Council Foundation (Grant Number 201708110129), the Beijing Natural Science Foundation (Grant Number 6173033), the National Natural Science Foundation of China (Grant Number 31570494), and the Beijing Municipal Commission of Education (Grant Numbers KM20131141 7007 and PXM2013_ 014209_ 07_ 000082). We also gratefully acknowledge the financial support of the Scientific Research Project from Facing Characteristic Discipline of Beijing Union University.

语种：英文

外文关键词：Rhodobacter sphaeroides; Antioxidant activities; Oxidative stress; Caco-2 cell

摘要：The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cells and intracellular cell-free extracts showed better antioxidant activities. Caco-2 cell-based oxidative stress model was developed by optimizing H2O2 concentration and culture time with the half lethal dose and methyl thiazolyl tetrazolium. By increasing the activity of endogenous antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, upregulating the antioxidant ability of the anti-superoxide anion and anti-hydroxyl radical, R. sphaeroides, especially the mutant strain R. sphaeroides (CGMCC No. 8513), exhibited significant protective activity against H2O2-induced oxidative stress in Caco-2 cells. Taken together, R. sphaeroides (CGMCC No. 8513) exhibits strong antioxidant activities and is a candidate to be investigated as a potential probiotic in the future.