文献类型：期刊文献

中文题名：高温蒸煮耦合木聚糖酶酶解麦麸制备低聚木糖条件优化

英文题名：Production of xylooligosaccharide from wheat bran by xylanase with autohydrolysis

作者：冀颐之[1];范光森[2];吴秋华[2];王洲[2];赵有玺[1];杜军[1,3,4]

第一作者：冀颐之

机构：[1]北京联合大学生物化学工程学院,生物质废弃物资源化利用北京市重点实验室,北京100023;[2]北京工商大学食品与健康学院,北京100048;[3]中国生物发酵产业协会,北京100833;[4]浙江华康药业股份有限公司,衢州324302

第一机构：北京联合大学生物化学工程学院生物医药系

年份：2020

卷号：40

期号：8

起止页码：1395-1402

中文期刊名：生命的化学

外文期刊名：Chemistry of Life

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

基金：浙江省引进培育领军型创新创业团队(2018R01014);北京联合大学人才强校优选计划(BPHR2017CZ06);生物质废弃物资源化利用北京市重点实验室开放课题。

语种：中文

中文关键词：麦麸;木聚糖酶;低聚木糖;高温蒸煮;正交试验;

外文关键词：wheat bran;xylanase;xylooligosaccharide;autohydrolysis;orthogonal experiment;

摘要：低聚木糖是一种效果优良的益生元,被广泛应用于各种食品中。以丰富的麦麸为原料,采用高温蒸煮耦合木聚糖酶处理制备低聚木糖具有较好的应用前景。本文以还原糖得率为指标,采用单因素实验分别研究了温度、转速、pH、酶浓度和酶解时间对木聚糖酶解效果的影响。随后,采用正交实验对温度、酶浓度和酶解时间进行组合优化,确定最佳酶解条件为:温度为65℃,转速为180 r/min,pH为5.5,酶浓度为105U/mL,酶解时间为4h。在最优条件下,还原糖得率为22.3%,低聚木糖得率为6.0%,主要由聚合度为2和3的低聚木糖组成,其比例分别为62.4%和24.9%。本文通过高温蒸煮耦合木聚糖酶对麦麸进行酶解处理制备低聚木糖具有一定的应用前景。
Xylooligosaccharide(XOS)is a prebiotic with good effect and is widely used in various foods.The preparation of XOS from wheat bran by autohydrolysis coupled with xylanase has a good prospect.In this study,the effects of temperature,rotation speed,pH,enzyme concentration and time on the yield of reducing sugar were studied by single factor experiment.Then,the temperature,enzyme concentration and time were optimized by orthogonal experiment.The optimal process conditions were obtained as follows:temperature was 65℃,rotational speed was 180 r/min,pH5.5,enzyme concentration was 105 U/mL,treatment time was 4 h.Under these optimized conditions,the yield of reducing sugars was 22.3%,and the yield of XOS was 6.0%.The XOS was mainly composed of XOS with degree of polymerization of two and three,and the proportions were 62.4%and 24.9%,respectively.In this paper,the preparation of XOS from wheat bran by autohydrolysis coupled with xylanase has a certain application prospect.