文献类型：期刊文献

中文题名：一种应用Asta-E-H脂肪酶定量检测雨生红球藻萃取物中虾青素的方法

英文题名：A method of detecting astaxanthin content in Haematococcus pluvialis extracted by lipase Asta-E-H

作者：高洁[1];宫平[1];惠伯棣[1];胡春凤[2];王旭[2];周牧函[1];杨楠[1]

第一作者：高洁

机构：[1]北京联合大学食品科学系;[2]秦皇岛惠恩生物技术有限公司

第一机构：北京联合大学生物化学工程学院食品科学系

年份：2018

卷号：0

期号：10

起止页码：93-102

中文期刊名：中国食品添加剂

外文期刊名：China Food Additives

收录：CSTPCD

语种：中文

中文关键词：雨生红球藻;虾青素酯;游离态虾青素;脂肪酶;水解;乳化;定量

外文关键词：Haematococcus pluvialis;astaxanthin ester;free astaxanthin;lipase;hydrolysis;emulsification;quantification

摘要：目的:建立定量检测雨生红球藻及其萃取物中虾青素含量的方法。方法:使用新型脂肪酶AstaE-H催化水解虾青素酯,再采用外标法在C30-HPLC上对游离态虾青素进行定量。结果:测定结果的RSD值达到2.63%,加样回收率为100.08%,检测下限为40ng/mL,线性范围为0.04～25μg/mL。结论:应用AstaE-H脂肪酶的方法具有重复性好、灵敏度和回收率高、反应副产物(如虾红素和顺式异构体)少等优点,优于国标GB/T31520-2015方法,且成本远低于美国药典方法,可在相关产业中推广应用。
Abstract ： Objective ： This study aims to develop a method for the quantification of astaxanthin content from Haematococcus pluvialis extracts. Method .. Astaxanthin esters from alga were hydrolyzed by a novel lipase catalysis of Asta-E-H. The astaxanthin was then quantified on C30-HPLC by external standard. Results ~ RSD of the method was 2.63% and the recovery rate was 100.08%. The detection limit was 40ng/mL, linear range was 0.04 - 25mg/ mL. Conclusion ~ The method with Asta-E-H has the advantages on satisfied reproducibility, high sensitivity and good recovery rate, and less by-products （eg. astacene and Z-isomer） compared with those of national standard GB/T31520-2015 ; the method costs less than U.S pharmacopoeia method. The method has potential to apply in the related fields.