文献类型：期刊文献

中文题名：高效液相色谱-串联质谱法分离鉴定类球红细菌红色素

英文题名：High performance liquid chromatography-mass spectrometry for analysis of red pigment from Rhodobacter sphaeroides

作者：李祖明[1];李健[1];惠伯棣[1];张猛[1];白志辉[2];高兆兰[1]

机构：[1]北京联合大学应用文理学院;[2]中国科学院生态环境研究中心

第一机构：北京联合大学应用文理学院

年份：2013

卷号：44

期号：8

起止页码：76-80

中文期刊名：东北农业大学学报

外文期刊名：Journal of Northeast Agricultural University

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：北京市教委2012年"启明星"国家级创新创业训练计划项目;北京市教委科技计划面上项目(KM201311417007)

语种：中文

中文关键词：反相高效液相色谱-串联质谱(RP—HPLC—ESI—MS;MS);C30柱;类球红细菌;红色素;类胡萝卜素

外文关键词：reverse phase high performance liquid chromatography-electrospray ionization tandem mass spectroscopy/mass spectroscopy（RP-HPLC-ESI-MS/MS）; C30 column; Rhodobacter sphaeroides; red pigment; carotenoids

摘要：利用反相高效液相色谱/二极管阵列检测器/电喷雾质谱联用技术研究类球红细菌红色素,分离鉴定出一种组分,属于类胡萝卜素。采用C30色谱柱,在甲醇-乙腈-MTBE(3 1 1,V/V/V)为流动相,流速为1 mL·min-1,检测波长为475 nm的色谱条件下,类球红细菌红色素中有11个色谱峰在55 min内得到显著分离。借助反相高效液相色谱-电子喷雾离子源质谱联用(RP-HPLC-ESI-MS/MS)法初步确鉴定出这11个色谱峰中一种主要组分,为Philosamiaxanthin,其相对分子质量为566.5。
One main compositions of red pigment from Rhodobacter sphaeroides, which belong to carotenoids, were isolated and identified using reverse phase high performance liquid chromatography （RP-HPLC） equipped with a C30 column and a PDA detector and characterized by UV spectrometry and electrospray ionization tandem mass spectrometry/mass spectroscopy （ESI-MS/MS）. As a ternary solvent system of methanol- methyl cyanide-methylt-butyl ether（MTBE） （3： 1： 1, VNN） was used as mobile phase, eleven chromatographic peaks of red pigment from Rhodobacter sphaeroides were resolved within 55 min with a C30 column of 1 mL/min flow rate at 475 nm detection. The fraction which were the main compositions of the eleven chromatographic peaks products, were identified as Philosamiaxanthin of carotenoids with the help of reverse phase high performance liquid chromatography-electrospray ionization tandem mass spectroscopy/mass spectroscopy（RP-HPLC-ESI-MS/MS）, and their relative molecular weight were 566.5.