文献类型：期刊文献

中文题名：姜黄素对过度训练大鼠骨骼肌Nrf2通路相关蛋白质表达及细胞凋亡的影响

英文题名：Effects of Curcumin on Nrf2 Pathway-associated Protein Expression and Apoptosis in Skeletal Muscle of Overtraining Rats

作者：牛衍龙[1];曹建民[2];王祯[2];周海涛[3,4];龚平[3];胡戈[5];田旭宸[3];邵芙蓉[3]

第一作者：牛衍龙

机构：[1]赣南医学院康复学院,中国江西赣州341000;[2]北京体育大学运动人体科学学院,中国北京100084;[3]北京联合大学生物化学工程学院,中国北京100023;[4]北京联合大学生物活性物质与功能食品北京市重点实验室,中国北京100191;[5]长治医学院,中国山西长治046000

第一机构：赣南医学院康复学院,中国江西赣州341000

年份：2020

卷号：24

期号：5

起止页码：367-375

中文期刊名：生命科学研究

外文期刊名：Life Science Research

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

基金：北京市朝阳区协同创新项目(CYXC1817);北京市高等学校高水平人才交叉培养“实培计划”项目;北京联合大学科研项目(ZK70202005);北京联合大学2019年“启明星”大学生科技创新创业项目(201911417SJ155);江西省教育厅科学技术研究项目(GJJ190800);赣南医学院博士启动基金课题(QD201817);江西省中医药科研课题(2019A073)。

语种：中文

中文关键词：姜黄素;过度训练;骨骼肌;细胞凋亡;核因子E2相关因子2(Nrf2)

外文关键词：curcumin;overtraining;skeletal muscle;apoptosis;Nrf2

摘要：为研究姜黄素对过度训练大鼠骨骼肌核因子E2相关因子2(nuclear factor-erythroid 2-related factor 2,Nrf2)通路相关蛋白质表达及细胞凋亡的影响,选取72只7周龄Wistar大鼠,将其分为安静组(C)、姜黄素组(CC)、过度训练模型组(OM)和姜黄素干预组(COM)。C组和CC组无运动干预,其他组进行8周递增负荷游泳运动。训练期间,CC组和COM组以200 mg/(kg·d)、5 mL/kg姜黄素灌胃,其他组以等体积0.5%羧甲基纤维素钠灌胃。末次训练结束24 h后取材,骨骼肌经HE染色后进行病理学诊断,采用TUNEL法检测骨骼肌细胞凋亡水平,采用免疫组化法、酶联免疫法、放射免疫法等方法检测相关生化指标,包括:血清睾酮(testosterone,T)、皮质酮(corticosterone,Cor)、肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactate dehydrogenase,LDH);骨骼肌超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、Nrf2、血红素氧合酶-1(heme oxygenase-1,HO-1)、B细胞淋巴瘤2(B cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2-associated X protein,Bax)。结果发现,与C组比较,CC组骨骼肌组织形态及各项生化指标均无显著性变化,而OM组骨骼肌组织形态发生病理学改变,血清T/Cor比值以及骨骼肌SOD、Nrf2、HO-1、Bcl-2水平和Bcl-2/Bax比值显著降低(P<0.05或P<0.01),血清CK、LDH水平以及骨骼肌的细胞凋亡水平、MDA和Bax含量显著增加(P<0.01);与OM组比较,COM组骨骼肌组织形态的病理学改变有所改善,血清T/Cor比值以及骨骼肌SOD、Nrf2、HO-1水平和Bcl-2/Bax比值显著升高(P<0.05或P<0.01),血清CK、LDH水平以及骨骼肌的细胞凋亡水平、MDA和Bax含量显著降低(P<0.05或P<0.01)。本研究初步证明8周递增强度游泳训练可引发大鼠过度训练,使骨骼肌氧化应激加剧,抗氧化能力下降,细胞凋亡增加,从而导致骨骼肌发生组织形态病理学改变和功能损伤;姜黄素可通过调控Nrf2通路相关蛋白质的表达,提高骨骼肌抗氧化酶活性及抗氧化能力,有效清除自由基,从而抑制和缓解细胞凋亡及过度训练所致结构和功能损伤。
To study the effects of curcumin on nuclear factor-erythroid 2-related factor 2(Nrf2)pathway-associated protein expression and apoptosis in skeletal muscle of overtraining rats,seventy-two 7-week-old Wistar rats were divided into control group(C),curcumin group(CC),overtraining model group(OM),and curcumin treatment combined with overtraining group(COM).There was no exercise intervention in C and CC groups,and the other groups underwent 8-week incremental load swimming.During the training period,the CC and COM groups were intragastrically administered with curcumin at a dose of 200 mg/(kg·d)and 5 mL/kg,and the other groups were intragastrically administered with an equal volume of 0.5%carboxymethylcellulose.All the rats were dissected 24 h after the last training,and skeletal muscle samples were taken for histopathological diagnosis after HE staining.The apoptosis of skeletal muscle cells was detected by TUNEL,and the related biochemical indexes were detected by immunohistochemistry,ELISA and radioimmunoassay.The biochemical indicators included testosterone(T),corticosterone(Cor),creatine kinase(CK)and lactate dehydrogenase(LDH)in serum,and superoxide dismutase(SOD),malondialdehyde(MDA),Nrf2,heme oxygenase-1(HO-1),Bcl-2,Bcl-2-associated X protein(Bax)in skeletal muscle.Compared with the C group,the CC group had no significant changes in histopathology of the skeletal muscle and biochemical indexes,but the OM group had significant pathological changes in the skeletal muscle,and the content of T/Cor in serum and the expressions of SOD,Nrf2,HO-1,Bcl-2 and Bcl-2/Bax in skeletal muscle were significantly decreased(P<0.05 or P<0.01),while the levels of CK and LDH in serum,and apoptosis level,MDA and Bax in skeletal muscle were increased significantly(P<0.01).Compared with the OM group,the pathological changes of skeletal muscle in COM group were improved,the content of T/Cor in serum,and the levels of SOD,Nrf2,HO-1 and Bcl-2/Bax in skeletal muscle were significantly increased(P<0.05 or P<0.01),while the levels of CK and LDH in serum,and apoptosis level,MDA and Bax in skeletal muscle were significantly decreased(P<0.05 or P<0.01).In conclusion,8-week incremental load swimming induced overtraining in rats,with increased oxidative stress and apoptosis of skeletal muscle,and decreased antioxidant capacity,leading to pathological changes and functional impairment of skeletal muscle.Moreover,the results indicate that curcumin can increase the antioxidant enzyme activity and antioxidant capacity of skeletal muscle by regulating the expression of Nrf2 pathway-related proteins,then scavenge free radicals,and alleviate structural and functional damages caused by apoptosis and overtraining.