文献类型：期刊文献

英文题名：Glycerol promotes biomass accumulation of Klebsiella pneumoniae by activating dha regulon

作者：Li, Ying[1];He, Runying[1];Ge, Xizhen[1]

第一作者：李映

通讯作者：Ge, XZ[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 10023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 10023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2022

卷号：117

起止页码：68-75

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000806816900007)】；

基金：Acknowledgement This work was financially supported by Beijing Municipal Education Commission Technology Plan [KM202011417006] .

语种：英文

外文关键词：Klebsiella pneumoniae; Glycerol; Dha regulon; Biomass accumulation; Transcriptome analysis

摘要：Biomass accumulation is a key factor for successful biosynthesis of chemicals in industrial scale. As an important platform for biorefinery, Klebsiella pneumoniae has been widely used for the production of various chemicals. However, low production of chemicals is often ascribed to insufficient biomass. Herein we demonstrated that activation of dha regulon can efficiently promote biomass accumulation of K. pneumoniae in both minimal and nutrient medium. Transcriptome analysis revealed that dha regulon was activated by supply of 5 g/L glycerol in glucose medium, and neither glycerol was consumed nor 1,3-propanediol was produced before glucose was exhausted. Moreover, biofilm formation was retarded but cell motility was accelerated. Interestingly, the susceptibility of K. pneumoniae to common antibiotics was not elevated. Next, mutation and overexpression of the genes in dha regulon were conducted and results demonstrated that loss of the regulator dhaR led to growth defect. On the contrary, overexpression of dhaD, dhaB, dhaR and dhaT contributed partly to the promoted biomass accumulation in glucose medium. In summary, activation of dha regulon of K. pneumoniae enhanced biomass accumulation but had little impact on antibiotic susceptibility. This study provided a promising method for improving the productivity of valuable chemicals in industrial scale.