文献类型：期刊文献

中文题名：毛细管电泳电致化学发光法测定家犬体内甲氧氯普胺的血药浓度

英文题名：Determination of Metoclopramide in Dog Plasma by Capillary Electrophoresis with Electrochemiluminescence

作者：周考文[1];顾雪梅[2];崔晓超[1];陈晓翠[1]

第一作者：周考文

通讯作者：Zhou, KW[1]

机构：[1]北京联合大学生物化学工程学院;[2]中国人民解放军北京军区总医院

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2011

卷号：69

期号：2

起止页码：221-225

中文期刊名：化学学报

外文期刊名：Acta Chimica Sinica

收录：CSTPCD;;Scopus(收录号：2-s2.0-84867053330);WOS:【SCI-EXPANDED(收录号:WOS:000287907800018)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：北京市教委科技发展计划(No.KM200711417005);北京市骨干教师人才强教计划资助项目

语种：中文

中文关键词：毛细管电泳;电化学发光;甲氧氯普胺;血药浓度

外文关键词：capillary electrophoresis; electrochemiluminescence; metoclopramide; plasma drug concentra-tion

摘要：基于甲氧氯普胺对钌联吡啶电化学发光信号的增敏作用,建立了检测甲氧氯普胺的毛细管电泳电致化学发光新方法.最佳实验条件为:检测电位1.18 V,钌联吡啶浓度5 mmol/L,检测池磷酸缓冲溶液40 mmol/L(pH 7.2),分离磷酸缓冲溶液20 mmol/L(pH 6.1),进样电压12 kV,进样时间10 s,分离电压14 kV.方法的检测限(3σ)为5.0×10-3 mg/L,线性范围为0.03～9.52 mg/L,相关系数为0.9990,回收率为98.0%～101.7%.对家犬体内甲氧氯普胺血药浓度的监测发现,给药1.5 h血药浓度达到最大值,血药浓度的半衰期约为4 h.本方法具有简便、快速、灵敏、进样量少等特点,可用于甲氧氯普胺血药浓度监控和药物动力学研究.
Based on the phenomenon that metoclopramide（MCL） could consumingly enhance the electro-chemiluminescence（ECL） intensity of tris（2,2＇-bipyridyl）ruthenium,a novel and sensitive method was proposed for determination of metoclopramide using capillary electrophoresis（CE） coupled with electrochemiluminescence（ECL） detection.The influence of several experiment parameters was ex-plored.The optimum experimental conditions were detection potential 1.18 V（vs.Ag/AgCl）,40 mmol/L of phosphate buffer（pH 7.2） containing 5 mmol/L in CEL detection cell,running buffer of 20 mmol/L（pH 6.1）,sample injection of 10 s at 12 kV,and separation voltage of 14 kV.The detection limit（3σ） of this method was 5.0×10-3 mg/L.The linear range of ECL intensity versus concentration of meto-clopramide was 0.03～9.52 mg/L,and the correlation coefficient was 0.9990.The recovery of testing 5 standard samples by this method was 98.0%～101.7%.Maximum of MCL concentration in Dog Plasma ap-peared at 1.5 h of drugging,and half life of the concentration was about 4 h.This method had the merits of simpleness,speediness,sensitivity,little sample injection,and could successfully avoid the influencing of interference.This method was successfully utilized to the assay of metoclopramide in dog plasma.