文献类型：期刊文献

中文题名：磁性壳聚糖微球固定化果胶酶的研究

英文题名：Study on Immobilization of Pectinase on Magnetic Chitosan Microspheres

作者：李鸿玉[1];厉重先[1];李祖明[1]

第一作者：李鸿玉

机构：[1]北京联合大学师范学院

第一机构：北京联合大学师范学院

年份：2008

卷号：29

期号：9

起止页码：399-403

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：壳聚糖;果胶酶;固定化

外文关键词：chitosan; pectinase; immobilization

摘要：以壳聚糖复合Fe3O4为载体,采用吸附-交联法固定化果胶酶。考察壳聚糖的脱乙酰度、分子量对微球载体形态的影响;对固定化过程中酶浓度、pH值、温度、时间等因素进行考察;采用正交试验设计对载体制备与酶固定化中的主要条件进行优化。结果表明,随着壳聚糖脱乙酰度、分子量的增加,载体表现出良好性能;脱乙酰度为95%、分子量为20万的壳聚糖浓度为3%、Fe3O4的加量与壳聚糖质量比为1:3、戊二醛浓度为3%、酶加量为0.3ml时,固定化果胶酶活力最高,其酶活保存率为79%。
In this experiment, taking the chitosan complexed with Fe304 as the carrier, the adsorption- crosslinking method was used to immobilize pectinase. Effects of deacetylation degree of chitosan and molecular weight on the shape of the cartier were studied, and effects of enzyme concentration, pH, temperature and time, etc. on the process of immobilization were also studied. The method oforthogonal test was used to optimize the main conditions of carrier preparation and enzyme immobilization. The results indicated that with the increases of the deacetylation degree of chitosan and its molecular weight, the carrier shows good performance. The optimum conditions for the activity of the deacetylation degree of the ehitosan 95% with 200000 molecular weight, its concentration 3%, Fe304 to chitosan ratio 1：3, concentration of glutaric dialdehyde 3%, and addition of pectinase as 0.3 ml, with preservation rate ofpectinase activity 79%.