文献类型：期刊文献

英文题名：In vitro evaluation of freeze-drying chitosan-mineralized collagen/Mg-Ca alloy composites for osteogenesis

作者：Zhang, Zhenbao[1];Sun, Xirao[1];Yang, Jingxin[2,3];Wang, Chengyue[1]

第一作者：Zhang, Zhenbao

通讯作者：Wang, CY[1];Yang, JX[2]

机构：[1]Jinzhou Med Univ, Dept Prosthodont, Affiliated Hosp 2, Jinzhou 121000, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Informat Serv Engn, Beijing, Peoples R China;[3]Beijing Union Univ, Coll Robot, Beijing, Peoples R China

第一机构：Jinzhou Med Univ, Dept Prosthodont, Affiliated Hosp 2, Jinzhou 121000, Peoples R China

通讯机构：[1]corresponding author), Jinzhou Med Univ, Dept Prosthodont, Affiliated Hosp 2, Jinzhou 121000, Peoples R China;[2]corresponding author), Beijing Union Univ, Beijing, Peoples R China.|[11417]北京联合大学;

年份：2022

卷号：36

期号：8

起止页码：1359-1377

外文期刊名：JOURNAL OF BIOMATERIALS APPLICATIONS

收录：;EI(收录号：20220311471666);Scopus(收录号：2-s2.0-85122724104);WOS:【SCI-EXPANDED(收录号:WOS:000746270300001)】；

基金：The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study is supported by Beijing Union University (ZK70202006), Department of Education of Liaoning Province (JYTZD2020004), Natural Science Foundation of Liaoning Province (2019-MS-141).

语种：英文

外文关键词：Mg-Ca alloy; mineralized collagen; chitosan; osteogensis; angiogenesis

摘要：Magnesium (Mg) alloy with good mechanical properties and biodegradability is considered as one of the ideal bone repair materials. However, the rapid corrosion of Mg-based metals can pose harm to the function of an implant in clinical applications. In this study, micro-arc oxidation coating was prepared on the surface of the Mg-Ca matrix, then the chitosan and mineralized collagen (nano-hydroxyapatite/collagen; nHAC) were immobilized on the surface of the MAO/Mg-Ca matrix to construct the CS-nHAC/Mg-Ca composites of different component proportions (the ratio of CS to nHAC is 2:1, 1:1, and 1:2, respectively). The corrosion resistance, osteogenic activity, and angiogenic ability were extensively investigated. The results indicated that the CS-nHAC reinforcement materials can improve the corrosion resistance of the Mg matrix significantly and promote the proliferation and adhesion of mouse embryo osteoblast precursor cells (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs). In addition, the CS-nHAC/Mg-Ca composites can not only promote the alkaline phosphatase (ALP) activity and extracellular matrix mineralization of MC3T3-E1 cells but also enhance the migration motility and vascular endothelial growth factor (VEGF) expression of HUVECs. Meanwhile, the 2CS-1nHAC/Mg-Ca composite exhibited the optimum function characteristics compared with other samples. Therefore, considering the improvement of corrosion resistance and biocompatibility, the CS-nHAC/Mg-Ca composites are expected to be a promising orthopedic implant.