文献类型：期刊文献

英文题名：Identification and antioxidant activity of carotenoids from superfine powder of Rhodobacter Sphaeroides

作者：Li, Zuming[1,2];Kong, Lina[1,2];Hui, Bodi[1,2];Shang, Xiaoya[1,2];Gao, Liping[1,2];Luan, Na[1,2];Zhuang, Xuliang[3,4];Wang, Dong[5];Bai, Zhihui[3,4]

第一作者：李祖明

通讯作者：Bai, ZH[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Beijing, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China;[3]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China;[4]Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China;[5]Wuxi Zhongke Huoli Biotechnol Co LTD, Yixing City, Jiangsu, Peoples R China

第一机构：北京联合大学生物化学工程学院食品科学系

通讯机构：[1]corresponding author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China.

年份：2017

卷号：29

期号：11

起止页码：833-845

外文期刊名：EMIRATES JOURNAL OF FOOD AND AGRICULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000418290700003)】；

基金：This paper was supported by the National Natural Science Foundation of China [grant number 3157020423 and 3157020721], China Scholarship Council Foundation [grant number 201708110129], Beijing Natural Science Foundation [grant number 6173033] and Beijing Municipal Commission of Education [grant number KM201311417007 and PXM2013_ 014209_07_000082], We also gratefully acknowledge financial support of Scientific Research Project from Facing Characteristic Discipline of Beijing Union University (grant number KYDE40201703).

语种：英文

外文关键词：Rhodobacter sphaeroides; Carotenoids; Superfine grinding; HPLC-DAD-APCI-MS; Antioxidant activity

摘要：The interest in carotenoids from the natural-antioxidant point of view has recently risen sharply because of their substantial health benefits. Here, we report the identification and antioxidant activity of carotenoids extracted by superfine grinding from Rhodobacter sphaeroides 3757 which is a species of photosynthetic bacteria. After separation and purification by silica gel column chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), the four major carotenoids from the superfine powder of R. sphaeroide 3757 were identified as bixin, hydroxyspheroidenone, 3,3,4 c-tetrahydrospirilloxanthin-20-al, and spheroidenone by reversed phase-high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (RP-HPLC-APCI-MS). The antioxidant activity of the carotenoids extracted after superfine grinding of dry biomass of R. sphaeroide 3757 was higher than that after ultrasonic treatment. When the ratio solvent-to-solid was 30, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, and lipid peroxidation inhibitory activity of the extracts after superfine grinding were 74.0% +/- 3.1%, 0.497 +/- 0.022, and 77.6% +/- 3.2%, respectively. By contrast, the extracts after ultrasonic treatment, these numbers were 61,0% +/- 2,5%, 0.328 +/- 0,01 4, and 55.2% +/- 2.3%, respectively. These results indicate that carotenoids from R. sphaeroide 3757 show a significant antioxidant activity in vitro in a concentration-dependent manner, and that superfine grinding is the optimal extraction method. We hope to provide scientific guidance for commercial production of natural-antioxidant and functional food from carotenoids of R. sphaeroide.