文献类型：期刊文献

英文题名：The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere

作者：Zhang, Baoguo[1];Bai, Zhihui[1];Hoefel, Daniel[2];Tang, Ling[1];Wang, Xiaoyi[3];Li, Baoju[4];Li, Zuming[5];Zhuang, Guoqiang[1]

第一作者：Zhang, Baoguo

通讯作者：Bai, ZH[1]

机构：[1]Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China;[2]S Australian Water Corp, Australian Water Qual Ctr, Bolivar, SA 5110, Australia;[3]Entomol Commonwealth Sci & Ind Res Org, Black Mt, ACT 2601, Australia;[4]Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China;[5]Beijing Union Univ, Teachers Coll, Beijing 100011, Peoples R China

第一机构：Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

通讯机构：[1]corresponding author), 18 Shuangqing Rd, Beijing 100085, Peoples R China.

年份：2009

卷号：407

期号：6

起止页码：1915-1922

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20090811915934);Scopus(收录号：2-s2.0-60049085108);WOS:【SCI-EXPANDED(收录号:WOS:000264357100011)】；

基金：This study was funded by the National Natural Science Foundation of China (No. 30600082), the "Knowledge innovation" Program of Chinese Academy of Sciences (kzcx1-yw-06-03) and the Beijing Municipal Commission of Education (20061D0502200295 and KM200811417006). We thank Prof Yehuda Cohen for his helpful suggestions.

语种：英文

外文关键词：Cypermethrin; Phyllosphere; Microbial community; PLFA; DGGE

摘要：Although pesticides have been extensively used for controlling insects and disease pathogens of plants, little is known regarding the impacts of applying these pesticides on the microbial community in the plant phyllosphere. Here, we report the effects of cypermethrin pesticide application upon the microbial community of the pepper plant phyllosphere. Assessments were made using culture-independent techniques including phospholipid fatty acid analysis (PLFA) and 16S rRNA gene directed Polymerase Chain Reaction with Denaturing Gradient Gel Electrophoresis (PCR-DGGE). During the 21 day greenhouse study, PLFA results indicated that both total and bacterial biomass increased after application of the pesticide. PLFA profiles also indicated that Gram-negative bacteria became predominant. DGGE analysis confirmed a significant change in bacterial community structure within the phyllosphere following the pesticide application where different dendrogram. clusters were observed between control and treated samples. Phylogenetic analysis also suggested a change in bacterial phyla following treatment, where bands sequenced within control cultures were predominantly of the Firmicutes phylum, but those bands sequenced in the treated samples were predominantly members of the Bacteroidetes and gamma-Proteobacteria phyla. In conclusion, this study revealed an increase in bacterial abundance and a shift in community composition within the pepper plant phyllosphere following the pesticide application, and highlighted the effective use of PLFA and PCR-DGGE for studying the effect of pesticides upon indigenous phyllosphere microbes. (C) 2008 Elsevier B.V. All rights reserved.