文献类型：期刊文献

中文题名：Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism

作者：Xiaojing Nie[1];Xirao Sun[1];Chengyue Wang[1];Jingxin Yang[2]

第一作者：Xiaojing Nie

机构：[1]Department of Prosthodontics,The Second Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China;[2]College of Robotics,Beijing Union University,Beijing 100000,China

第一机构：Department of Prosthodontics,The Second Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China

年份：2020

卷号：7

期号：1

起止页码：53-61

中文期刊名：再生生物材料（英文版）

收录：PubMed

基金：This work was supported by Science and Technology Fund of Liaoning Province(No.20180530071 and No.2019-MS-141).

语种：英文

中文关键词：magnesium ion;integrin;Type I collagen;FAK/ERK

摘要：Type I collagen(Col I)is a main component of extracellular matrix(ECM).Its safety,biocompatibility,hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications.Mg2t also control a myriad of cellular processes,including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing.In our studies,Mg2t bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways.In order to clarify the biological behavior effect of 10mM Mg2t/Col I coating,we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),alkaline phosphatase(ALP),406-diamidino-2-phenylindole(DAPI),Alizarin red staining and Rhodamine B-isothiocyanate(RITC)-labeled phalloidin experiments and found that 10mM Mg2t group,Col I-coating group,10mM Mg2t/Col I-coating group,respectively,promoted the proliferation and differentiation of osteoblasts,especially 10mM Mg2t/Col I-coating group.We detected the mRNA expression of osteogenic-related genes(Runx2,ALP and OCN,OPN and BMP-2)and the protein expression of signaling pathway(integrin a2,integrin b1,FAK and ERK1/2),these results indicated that 10mM Mg2t/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity.The potential mechanisms of this specific performance may be through activating via integrin a2b1-FAK-ERK1/2 protein-coupled receptor pathway.